scispace - formally typeset
Search or ask a question
Author

Cayla Baynes

Bio: Cayla Baynes is an academic researcher from University of Arizona. The author has contributed to research in topics: Fascin & Genetic model. The author has an hindex of 3, co-authored 4 publications receiving 61 citations.
Topics: Fascin, Genetic model, Drug discovery, Cancer, Antigen

Papers
More filters
Journal ArticleDOI
TL;DR: The image processing and PCA procedure can be implemented as a stand-alone smartphone application and can be adopted as an extremely low-cost, disposable, fully handheld, easy-to-use, yet sensitive and specific quality control method for appraising red wine or similar beverage products in resource-limited environments.
Abstract: A paper microfluidic chip was designed and fabricated to evaluate the taste of 10 different red wines using a set of chemical dyes. The digital camera of a smartphone captured the images, and its red-green-blue (RGB) pixel intensities were analyzed by principal component analysis (PCA). Using 8 dyes and 2 principal components (PCs), we were able to distinguish each wine by the grape variety and the oxidation status. Through comparing with the flavor map by human evaluation, PC1 seemed to represent the sweetness and PC2 the bodyness of red wine. This superior performance is attributed to: (1) careful selection of commercially available dyes through a series of linear correlation studies with the taste chemicals in red wines, (2) minimization of sample-to-sample variation by splitting a single sample into multiple wells on the paper microfluidics, and (3) filtration of particulate matter through paper fibers. The image processing and PCA procedure can eventually be implemented as a stand-alone smartphone application and can be adopted as an extremely low-cost, disposable, fully handheld, easy-to-use, yet sensitive and specific quality control method for appraising red wine or similar beverage products in resource-limited environments.

37 citations

Journal ArticleDOI
TL;DR: Evidence is provided that primary neuron culture using a genetic model organism can be valuable for early-stage drug discovery and developmental neurotoxicity testing and it is proposed that bidirectional screening for brain-development disorders and invasive cancers represents an efficient, multipurpose strategy for drug discovery.
Abstract: SUMMARY The actin-bundling protein fascin is a key mediator of tumor invasion and metastasis and its activity drives filopodia formation, cell-shape changes and cell migration. Small-molecule inhibitors of fascin block tumor metastasis in animal models. Conversely, fascin deficiency might underlie the pathogenesis of some developmental brain disorders. To identify fascin-pathway modulators we devised a cell-based assay for fascin function and used it in a bidirectional drug screen. The screen utilized cultured fascin-deficient mutant Drosophila neurons, whose neurite arbors manifest the 'filagree' phenotype. Taking a repurposing approach, we screened a library of 1040 known compounds, many of them FDA-approved drugs, for filagree modifiers. Based on scaffold distribution, molecular-fingerprint similarities, and chemical-space distribution, this library has high structural diversity, supporting its utility as a screening tool. We identified 34 fascin-pathway blockers (with potential anti-metastasis activity) and 48 fascin- pathway enhancers (with potential cognitive-enhancer activity). The structural diversity of the active compounds suggests multiple molecular targets. Comparisons of active and inactive compounds provided preliminary structure-activity relationship information. The screen also revealed diverse neurotoxic effects of other drugs, notably the 'beads-on-a-string' defect, which is induced solely by statins. Statin-induced neurotoxicity is enhanced by fascin deficiency. In summary, we provide evidence that primary neuron culture using a genetic model organism can be valuable for early-stage drug discovery and developmental neurotoxicity testing. Furthermore, we propose that, given an appropriate assay for target-pathway function, bidirectional screening for brain-development disorders and invasive cancers represents an efficient, multipurpose strategy for drug discovery.

27 citations

Journal ArticleDOI
01 Feb 2018
TL;DR: The results with undiluted serum showed the larger dynamic range and smaller standard errors, which can be attributed to the presence of serum proteins, functioning as a stabilizer or a passivating protein for the particles within paper fibers.
Abstract: A microfluidic paper analytical device (μPAD) was created for the sensitive quantification of cancer antigens, carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA 19-9), from human who...

7 citations

Journal ArticleDOI
TL;DR: In this paper, the authors used a cell-based assay for fascin function and used it in a bidirectional drug screen to identify fascin-pathway modulators and identified 34 pathway blockers and 48 pathway enhancers with potential cognitive-enhancer activity.
Abstract: SUMMARY The actin-bundling protein fascin is a key mediator of tumor invasion and metastasis and its activity drives filopodia formation, cell-shape changes and cell migration. Small-molecule inhibitors of fascin block tumor metastasis in animal models. Conversely, fascin deficiency might underlie the pathogenesis of some developmental brain disorders. To identify fascin-pathway modulators we devised a cell-based assay for fascin function and used it in a bidirectional drug screen. The screen utilized cultured fascin-deficient mutant Drosophila neurons, whose neurite arbors manifest the 'filagree' phenotype. Taking a repurposing approach, we screened a library of 1040 known compounds, many of them FDA-approved drugs, for filagree modifiers. Based on scaffold distribution, molecular-fingerprint similarities, and chemical-space distribution, this library has high structural diversity, supporting its utility as a screening tool. We identified 34 fascin-pathway blockers (with potential anti-metastasis activity) and 48 fascin- pathway enhancers (with potential cognitive-enhancer activity). The structural diversity of the active compounds suggests multiple molecular targets. Comparisons of active and inactive compounds provided preliminary structure-activity relationship information. The screen also revealed diverse neurotoxic effects of other drugs, notably the 'beads-on-a-string' defect, which is induced solely by statins. Statin-induced neurotoxicity is enhanced by fascin deficiency. In summary, we provide evidence that primary neuron culture using a genetic model organism can be valuable for early-stage drug discovery and developmental neurotoxicity testing. Furthermore, we propose that, given an appropriate assay for target-pathway function, bidirectional screening for brain-development disorders and invasive cancers represents an efficient, multipurpose strategy for drug discovery.

6 citations


Cited by
More filters
28 Jul 2005
TL;DR: PfPMP1)与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作�ly.
Abstract: 抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1(PfPMP1)与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员,通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

18,940 citations

Journal ArticleDOI
20 Jun 2017-Sensors
TL;DR: This paper reviews the recent advancements and developments in the field of smartphone-based food diagnostic technologies, with an emphasis on custom modules to enhance smartphone sensing capabilities, and provides a systematic classification according to the detection strategy, providing a critical discussion of strengths and weaknesses.
Abstract: A new generation of mobile sensing approaches offers significant advantages over traditional platforms in terms of test speed, control, low cost, ease-of-operation, and data management, and requires minimal equipment and user involvement. The marriage of novel sensing technologies with cellphones enables the development of powerful lab-on-smartphone platforms for many important applications including medical diagnosis, environmental monitoring, and food safety analysis. This paper reviews the recent advancements and developments in the field of smartphone-based food diagnostic technologies, with an emphasis on custom modules to enhance smartphone sensing capabilities. These devices typically comprise multiple components such as detectors, sample processors, disposable chips, batteries and software, which are integrated with a commercial smartphone. One of the most important aspects of developing these systems is the integration of these components onto a compact and lightweight platform that requires minimal power. To date, researchers have demonstrated several promising approaches employing various sensing techniques and device configurations. We aim to provide a systematic classification according to the detection strategy, providing a critical discussion of strengths and weaknesses. We have also extended the analysis to the food scanning devices that are increasingly populating the Internet of Things (IoT) market, demonstrating how this field is indeed promising, as the research outputs are quickly capitalized on new start-up companies.

200 citations

Journal ArticleDOI
TL;DR: The overview of the different materials (glass, silicon, polymer, paper, and techniques for the fabrication of MF based POC devices along with their wide range of biosensor applications is presented.
Abstract: Point-of-care (POC) diagnostic devices have been predicted to provide a boon in health care especially in the diagnosis and detection of diseases. POC devices have been found to have many advantages like a rapid and precise response, portability, low cost, and non-requirement of specialized equipment. The major objective of a POC diagnostic research is to develop a chip-based, self-containing miniaturized device that can be used to examine different analytes in complex samples. Further, the integration of microfluidics (MF) with advanced biosensor technologies is likely to result in improved POC diagnostics. This paper presents the overview of the different materials (glass, silicon, polymer, paper) and techniques for the fabrication of MF based POC devices along with their wide range of biosensor applications. Besides this, the authors have presented in brief the challenges that MF is currently facing along with possible solutions that may result in the availability of the accessible, reliable, and cost-efficient technology. The development of these devices requires the combination of developed MF components into POC devices that are user-friendly, sensitive, stable, accurate, low cost, and minimally invasive. These MF based POC devices have tremendous potential in providing improved healthcare including easy monitoring, early detection of disease, and increased personalization.

181 citations

Journal ArticleDOI
TL;DR: In this review, recent research in smartphone chemical and biosensing is assessed, and discussion is made regarding the opportunities that new research methods have to improve the scope of resource-limited sensing.
Abstract: Developments in the emerging fields of smartphone chemical and biosensing have dovetailed with increased interest in environmental and health monitoring for resource-limited environments, culminating in research toward field-ready smartphone sensors. Optical sensors have been a particular focus, in which smartphone imaging and on-board analysis have been integrated into both existing and novel colorimetric, fluorescent, chemiluminescent, spectroscopy-based, and scattering-based assays. Research in recent years has shown promising progress, but substantial limitations still exist due to environmental lighting interference, reliance upon proprietary smartphone attachments, and the undefined sensitivity variations between different smartphones. In this review, recent research in smartphone chemical and biosensing is assessed, and discussion is made regarding the opportunities that new research methods have to improve the scope of resource-limited sensing.

156 citations

Journal ArticleDOI
TL;DR: The progress in functionalization of cellulose papers with antibodies, nucleic acids and nanomaterials in PBBs and μPADs, is discussed and critically evaluated.

131 citations