Charles J. Waechter

TL;DR: The current understanding of the enzymes involved in the de novo biosynthesis of Dol-P and other polyisoprenoid glycosyl carrier lipids is reviewed and the role of membrane proteins and enzymes that could be involved inThe transbilayer movement of the lipid intermediates and the recycling of Dol

TL;DR: A novel form of cell-free incubation involving in vitro and in situ labeling with GDP-[14C]Man of the polyprenyl-P-mannoses and the simpler PIMs of mycobacterial membranes demonstrated the synthesis of a novel α(1→6)-linked linear form of LM at the expense of the C35/C50-P -Man.

TL;DR: A novel CDG type, CDG‐Ij, is identified, resulting from deficiency in UDP‐GlcNAc: dolichol phosphate N‐acetyl‐glucosamine‐1 phosphate transferase (GPT) activity encoded by DPAGT1, which is concluded to be responsible for the CDG symptoms in this patient.

TL;DR: Results indicate that LPP1 and DPP1 account for most of the hydrolytic activities toward isoprenoid pyrophosphates, dolichyl-P-P, farnesyl-F-P -P, and geranylgeranyl-p-P but also suggest that yeast contain other enzymes capable of dephosphorylating these essential isopranoid intermediates.