C
Charlotte Kaplan
Researcher at ETH Zurich
Publications - 8
Citations - 638
Charlotte Kaplan is an academic researcher from ETH Zurich. The author has contributed to research in topics: Septin & Photoactivated localization microscopy. The author has an hindex of 5, co-authored 7 publications receiving 565 citations. Previous affiliations of Charlotte Kaplan include University of Zurich.
Papers
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Journal ArticleDOI
A simple, versatile method for GFP-based super-resolution microscopy via nanobodies
TL;DR: This work developed a method to use any GFP-tagged construct in single-molecule super-resolution microscopy by targeting GFP with small, high-affinity antibodies coupled to organic dyes and achieved nanometer spatial resolution and minimal linkage error when analyzing microtubules, living neurons and yeast cells.
Journal ArticleDOI
Optimized sample preparation for single-molecule localization-based superresolution microscopy in yeast
Charlotte Kaplan,Helge Ewers +1 more
TL;DR: An optimized sample preparation protocol is described that enables single-molecule localization microscopy at high resolution combined with improved structural preservation in Saccharomyces cerevisiae.
Journal ArticleDOI
Absolute Arrangement of Subunits in Cytoskeletal Septin Filaments in Cells Measured by Fluorescence Microscopy
Charlotte Kaplan,Bo Jing,Christian M. Winterflood,Andrew A. Bridges,Patricia Occhipinti,Jürgen J. Schmied,Sören Grinhagens,Thomas Gronemeyer,Philip Tinnefeld,Amy S. Gladfelter,Jonas Ries,Helge Ewers +11 more
TL;DR: The organization of subunits in cytoskeletal polymers in cells is resolved by light microscopy and cellular septins were resolved as localization pairs and thin stretches of equidistant localizations.
Book ChapterDOI
Localization microscopy in yeast.
TL;DR: This chapter provides general guidelines for localization microscopy with a focus on Saccharomyces cerevisiae, whose different cellular architecture complicates efforts to directly transfer protocols established in mammalian cells to yeast.
Journal ArticleDOI
Functional Redundancy of Septin Homologs in Dendritic Branching.
TL;DR: The experimental paradigm of dendritic branching in hippocampal rat neurons is used to ask, to what extent septins of the SEPT2-group are functionally redundant and provides a basis for the study of septin-specific functions in cells.