Christian Schwerk

TL;DR: The choroid plexus is the source of cerebrospinal fluid production and location of the blood-CSF barrier (BCSFB), which is constituted by the epithelial cells of the CP which responds to this challenge by the production of chemokines and cytokines as well as alterations of the barrier function of the BCSFB.

TL;DR: This review illustrates examples of established brain barrier models, in which the specific reaction patterns of different viral families can be analyzed, and highlights the pathogen specific array of cytokines and chemokines involved in immunological responses in viral CNS infections.

TL;DR: A functional human BCSFB model based on human choroid plexus papilloma cells (HIBCPP) is described for the first time, which display typical hallmarks of a B CSFB as the expression of junctional proteins and formation of tight junctions, a high electrical resistance and minimal levels of macromolecular flux when grown on transwell filters.

TL;DR: The development of an ‘inverted’ Transwell filter system of PCPEC enables the first time to investigate bacterial invasion and translocation from the physiologically relevant basolateral (blood) to the apical (cerobrospinal fluid) side and underline the relevance of the blood–cerebrosp spinal fluid barrier as a gate for bacterial entry into the central nervous system.

TL;DR: A porcine and human “inverted” culture insert system that enables leukocyte transmigration specifically from the physiologically relevant basolateral side and facilitates the investigation of leukocytes entry into the CNS via the blood-CSF barrier is developed.