Chuan Nan Luo

Bio: Chuan Nan Luo is an academic researcher from University of Jinan. The author has contributed to research in topics: Detection limit & Chemistry. The author has an hindex of 2, co-authored 4 publications receiving 13 citations.

Efficient ABEI-Dissolved O2-Ce(III, IV)-MOF Ternary Electrochemiluminescent System Combined with Self-Assembled Microfluidic Chips for Bioanalysis.

Abstract: A signal-amplified electrochemiluminescent (ECL) sensor chip was developed for sensitive analysis of procalcitonin (PCT). Herein, we first prepared a self-enhanced luminophore, which enhanced ECL responses through intramolecular reactions. Second, Au-Pd bimetallic nanocrystals and mixed-valence Ce-based metal-organic frameworks (MOFs) were introduced as co-reaction promoters to facilitate the reduction of dissolved O2. Based on the synergistic catalysis of Au and Pd, the spontaneous cyclic reaction of Ce(III)/Ce(IV), and the high electrochemical active surface area of Ce(III, IV) MOF, a large number of superoxide anion radicals (O2•-) and hydroxyl radicals (OH•) were produced. Therefore, the luminescence efficiency of N-(aminobutyl)-N-(ethylisoluminol)-dissolved O2 (ABEI-O2) systems were greatly improved, providing a new prospect for the application of dissolved O2 in ECL analysis. In addition, the affinity peptide ligands were used for the directional connection of antibodies to provide protection for the bioactivity of the proposed sensor. Finally, the microfluidic technology was applied to ECL analysis to integrate the three-electrode detection system into the self-assembled microfluidic chip, which realized the automation and portability of the detection process. The developed sensor showed high sensitivity for PCT detection with a detection limit of 3.46 fg/mL, which possessed positive significance for the clinical diagnosis of sepsis.

Molecularly Imprinted Polymer for Preparation and Adsorption of Phenylalanine

Abstract: Molecular imprinting technology was employed to produce one kind of Phenylalanine ( Phe) molecularly imprinted polymers (MIP)by precipitation polymerization using Phe, anhydrous alcohol, acrylamide, ethylene glycol dimethacrylate, and azobisisobutyronitrile as template, porogen, functional monomer, cross-linker and initiator respectively. In this study, the polymerization conditions were optimized. The template on particle size and morphology of polymers were investigated in detail by scanning electron microscopy (SEM) and adsorption isotherm determination. The binding properties of Phe on imprinted polymers were evaluated in water by equilibrium rebinding experiments, and the maximum number of adsorption was 0.60 mmol/g. It is indicated that the existence of binding sites in imprinted polymers was proved and the binding sites showed good specific and selective capability to the template molecule Phe. So the polymers would be used to separate Phe from medicine and food.

Highly Efficient PTCA/Co3O4/CuO/S2O82- Ternary Electrochemiluminescence System Combined with a Portable Chip for Bioanalysis.

Abstract: Herein, we reported an efficient electrochemiluminescence (ECL) biosensor chip for sensitive detection of neuron-specific enolase (NSE). First, 3,4,9,10-perylenetetracarboxylic acid with good luminescence characteristics was used as a luminophore to obtain a stable ECL signal. Subsequently, hollow porous Co3O4/CuO concave polyhedron nanocages (CPNCs) were designed as co-reaction promoters to amplify the luminescence signals for highly sensitive trace detection of NSE. In brief, the rapid cyclic conversion of Co3+/Co2+ and Cu2+/Cu+ redox pairs could continuously catalyze the reduction of persulfate (S2O82-), thus providing a large number of essential active intermediates (SO4•-) for ECL emission. Meanwhile, the unique structure of Co3O4/CuO CPNCs possessed a large specific surface area, which greatly improved its catalytic efficiency. Third, NKFRGKYKC was developed as an affinity ligand for specific antibody fixation, which improved incubation efficiency and protected bioactivity of antibodies. Finally, we independently designed a microchip and applied it for ECL detection to improve the practical application ability of the sensor. The developed biosensor exhibited good sensitivity with a wide linear range (10 fg/mL to 100 ng/mL) and a low detection limit (3.42 fg/mL), which played an active role in the clinical application of sensing analysis.

Molecularly Imprinted Polymer Prepared by Precipitation Polymerization for Quercetin

Abstract: In this paper, molecular imprinted polymer for quercetin was synthesized by precipitation polymerization with acrylamide as functional monomer, ethylene glycol dimethacrylate as cross-linker, 2,2-azobisisobutyronitrile as initiator and acetone as the solvent. The polymers’ adsorption properties were characterized. As results shown that the synthesized imprinted polymer is microspheres with particle size in the nanometer scale, the adsorption capacity of quercetin imprinted polymers is 1.1×10-5mol/g, adsorption temperature is 60°C, adsorption time is 30 min. The molecular imprinting technology was introduced in interference test with a satisfactory result in selectivity.

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011.

Abstract: Herein, we present a survey of the literature covering the development of molecular imprinting science and technology over the years 2004-2011. In total, 3779 references to the original papers, rev ...

Dyes and Pigments

Abstract: Colour Chemistry By R L M Allen Pp xii+336 (Thomas Nelson and Sons: London, 1971) £5

Flow injection chemiluminescence sensor based on core–shell magnetic molecularly imprinted nanoparticles for determination of chrysoidine in food samples

Abstract: A novel flow injection chemiluminescence (CL) sensor for the determination of chrysoidine (CSD) using magnetic molecularly imprinted polymers (MMIPs) as recognition element is reported. The MMIPs were polymerized at the surface of Fe3O4@SiO2 magnetic nanoparticles (MNPs) using methyl methacrylate (MAA) and ethylene glycol dimethacrylate (EGDMA) as functional monomer and cross-linking agent, respectively. The MMIPs were characterized by TEM, XRD, dynamic adsorption and static adsorption tests. Because of the imprinted sites almost situated at the surface of imprinting products, the MMIPs had excellent adsorption capacity, specific recognition and direct magnetic separation in samples. Then the synthesized CSD-MMIPs were employed as recognition element by packing into flow cell to establish a new flow injection CL sensor. The CL intensity responded linearly to the concentration of CSD in the range of 1.0 × 10−4–2.0 × 10−6 mol L−1 with a detection limit of 6.19 × 10−7 mol L−1. The relative standard deviation (RSD) for the determination of 2.0 × 10−6 mol L−1 of CSD was 2.3% (n = 11). The sensor is reusable and has a great improvement in sensitivity and selectivity for CL analysis. As a result, the novel MMIP-CL sensor had been successfully applied to the determination of CSD in food samples.