In the last decade, consistent and successful innovations have been achieved in the field of lasers and optics, collectively known as ‘photonics’, founding new applications in biomedicine, including clinical biopsy. Non-invasive photonics-based diagnostic modalities are rapidly expanding, and with their exponential improvement, there is a great potential to develop practical instrumentation for automatic detection and identification of different types and/or sub-types of diseases at a very early stage. While using conventional light for the studies of different properties of objects in materials science, astrophysics and biomedicine already has a long history, the interaction of polarized light and optical angular momentum with turbid tissue-like scattering media has not yet been ultimately explored. Since recently this research area became a hot topic. This feature issue is a first attempt to summarize the recognitions achieved in this emerging research field of polarized light and optical angular momentum for practical biomedical applications during the last years.

Polarization and Orbital Angular Momentum of Light in Biomedical Applications: feature issue introduction.

Abstract Using a novel variant of polarized light microscopy for high-contrast imaging and quantification of unstained histology slides, the current study assesses the prognostic potential of peri-tumoral collagenous stroma architecture in 32 human stage III colorectal cancer (CRC) patient samples. We analyze three distinct polarimetrically-derived images and their associated texture features, explore different unsupervised clustering algorithm models to group the data, and compare the resultant groupings with patient survival. The results demonstrate an appreciable total accuracy of ~ 78% with significant separation (p &lt; 0.05) across all approaches for the binary classification of 5-year patient survival outcomes. Surviving patients preferentially belonged to Cluster 1 irrespective of model approach, suggesting similar stromal microstructural characteristics in this sub-population. The results suggest that polarimetrically-derived stromal biomarkers may possess prognostic value that could improve clinical management/treatment stratification in CRC patients. 

https://www.nature.com/articles/s41598-022-16178-3.pdf

Polarimetric biomarkers of peri-tumoral stroma can correlate with 5-year survival in patients with left-sided colorectal cancer

Optical polarimetry is an emerging modality that effectively quantifies the bulk optical properties that correlate with the anisotropic structural properties of cardiac tissues. We demonstrate the application of a polarimetric tool for characterizing healthy and fibrotic human myocardial tissues efficiently with a high degree of accuracy.The study was aimed to characterize the myocardial tissues from the left ventricle and right ventricle of N = 7 control and N = 10 diseased subjects. The diseased subjects were composed of two groups: N = 7 with rheumatic heart disease (RHD) and N = 3 with myxomatous valve (MV) disease.A portable, affordable, and accurate linear polarization-based diagnostic tool is developed to measure the degree of linear polarization (DOLP) of the myocardial tissues while working at a wavelength of 850 nm.The sensitivity, specificity, and accuracy of the polarimetric tool in distinguishing the control group from the RHD group were found to be 73.33%, 76.92%, and 75%, respectively, and from the MV group were 91.6%, 62.5%, and 80%, respectively, which demonstrates the efficacy of the polarimetric tool to distinguish the healthy myocardial tissues from diseased tissues.We have successfully developed a polarimetric tool that can aid cardiologists in characterizing the myocardial tissues in conjunction with endomyocardial biopsy. This work should be followed up with experiments on a large cohort of control and diseased subjects. We intend to create and develop a probe to quantify the DOLP of in vivo heart tissue during surgery. 

Toward the development of a polarimetric tool to diagnose the fibrotic human ventricular myocardium

Polarimetric techniques are widely used in a vast number of applications such as remote sensing, material characterization, astronomy and biological tissue inspection. In this last scenario, different polarimetric observables have proved their potential for enhancing imaging visualization. In this work we use a set of polarimetric observables derived from the arrow decomposition of the Mueller matrix for the first time: enpolarizing, retarding and depolarizing descriptors. In particular, the mean intensity coefficient and the three indices of polarimetric purity, the absolute values and Poincaré orientations of diattenuation, polarizance, entrance retardance and exit retardance vectors are considered. Results show images with enhanced visualization or even revealing invisible structures when compared to standard intensity images. In particular, thanks to these metrics, we improve the visualization of the necrotic areas of a Vitis rupestris leaf. In the case of animal samples, boundaries between different fascicles inside a tendon of an ex vivo chicken sample are revealed, as is the directionality of fiber tracts of the subcortical white matter in an ex vivo cow brain. The experimental results show the potential for biophotonics imaging and how polarimetric techniques could be useful for biomedical and botanical applications.

Polarimetric Images of Biological Tissues Based on the Arrow Decomposition of Mueller Matrices

Deciphering the Morphology of Tumor-stromal Features in Invasive Breast Cancer Using Artificial Intelligence.

The tumour-stroma ratio (TSR) has been explored as a useful source of prognostic information in various cancers, including colorectal, breast, and gastric. Despite research showing potential prognostic utility, its uptake into the clinic has been limited, in part due to challenges associated with subjectivity, reproducibility, and quantification. We have recently proposed a simple, robust, and quantifiable high-contrast method of imaging intra- and peri-tumoural stroma based on polarized light microscopy. Here we report on its use to quantify TSR in human breast cancer using unstained slides from 40 patient samples of invasive ductal carcinoma (IDC). Polarimetric results based on a stromal abundance metric correlated well with pathology designations, showing a statistically significant difference between high- and low-stroma samples as scored by two clinical pathologists. The described polarized light imaging methodology shows promise for use as a quantitative, automatic, and standardizable tool for quantifying TSR, potentially addressing some of the challenges associated with its current estimation.

Ciara Murray

Papers

Toward a quantitative method for estimating tumour-stroma ratio in breast cancer using polarized light microscopy