https://www.massey.ac.nz/~ychisti/Biodiesel.pdf

Biodiesel from microalgae.

Sustainable production of renewable energy is being hotly debated globally since it is increasingly understood that first generation biofuels, primarily produced from food crops and mostly oil seeds are limited in their ability to achieve targets for biofuel production, climate change mitigation and economic growth. These concerns have increased the interest in developing second generation biofuels produced from non-food feedstocks such as microalgae, which potentially offer greatest opportunities in the longer term. This paper reviews the current status of microalgae use for biodiesel production, including their cultivation, harvesting, and processing. The microalgae species most used for biodiesel production are presented and their main advantages described in comparison with other available biodiesel feedstocks. The various aspects associated with the design of microalgae production units are described, giving an overview of the current state of development of algae cultivation systems (photo-bioreactors and open ponds). Other potential applications and products from microalgae are also presented such as for biological sequestration of CO 2 , wastewater treatment, in human health, as food additive, and for aquaculture.

/pdf/microalgae-for-biodiesel-production-and-other-applications-a-54vmwufrsu.pdf

Microalgae for biodiesel production and other applications: A review

Sustainability is a key principle in natural resource management, and it involves operational efficiency, minimisation of environmental impact and socio-economic considerations; all of which are interdependent. It has become increasingly obvious that continued reliance on fossil fuel energy resources is unsustainable, owing to both depleting world reserves and the green house gas emissions associated with their use. Therefore, there are vigorous research initiatives aimed at developing alternative renewable and potentially carbon neutral solid, liquid and gaseous biofuels as alternative energy resources. However, alternate energy resources akin to first generation biofuels derived from terrestrial crops such as sugarcane, sugar beet, maize and rapeseed place an enormous strain on world food markets, contribute to water shortages and precipitate the destruction of the world's forests. Second generation biofuels derived from lignocellulosic agriculture and forest residues and from non-food crop feedstocks address some of the above problems; however there is concern over competing land use or required land use changes. Therefore, based on current knowledge and technology projections, third generation biofuels specifically derived from microalgae are considered to be a technically viable alternative energy resource that is devoid of the major drawbacks associated with first and second generation biofuels. Microalgae are photosynthetic microorganisms with simple growing requirements (light, sugars, CO 2 , N, P, and K) that can produce lipids, proteins and carbohydrates in large amounts over short periods of time. These products can be processed into both biofuels and valuable co-products. This study reviewed the technologies underpinning microalgae-to-biofuels systems, focusing on the biomass production, harvesting, conversion technologies, and the extraction of useful co-products. It also reviewed the synergistic coupling of microalgae propagation with carbon sequestration and wastewater treatment potential for mitigation of environmental impacts associated with energy conversion and utilisation. It was found that, whereas there are outstanding issues related to photosynthetic efficiencies and biomass output, microalgae-derived biofuels could progressively substitute a significant proportion of the fossil fuels required to meet the growing energy demand.

/pdf/biofuels-from-microalgae-a-review-of-technologies-for-1t1wwr5e69.pdf

Biofuels from microalgae—A review of technologies for production, processing, and extractions of biofuels and co-products

The use of fossil fuels is now widely accepted as unsustainable due to depleting resources and the accumulation of greenhouse gases in the environment that have already exceeded the “dangerously high” threshold of 450 ppm CO2-e. To achieve environmental and economic sustainability, fuel production processes are required that are not only renewable, but also capable of sequestering atmospheric CO2. Currently, nearly all renewable energy sources (e.g. hydroelectric, solar, wind, tidal, geothermal) target the electricity market, while fuels make up a much larger share of the global energy demand (∼66%). Biofuels are therefore rapidly being developed. Second generation microalgal systems have the advantage that they can produce a wide range of feedstocks for the production of biodiesel, bioethanol, biomethane and biohydrogen. Biodiesel is currently produced from oil synthesized by conventional fuel crops that harvest the sun’s energy and store it as chemical energy. This presents a route for renewable and carbon-neutral fuel production. However, current supplies from oil crops and animal fats account for only approximately 0.3% of the current demand for transport fuels. Increasing biofuel production on arable land could have severe consequences for global food supply. In contrast, producing biodiesel from algae is widely regarded as one of the most efficient ways of generating biofuels and also appears to represent the only current renewable source of oil that could meet the global demand for transport fuels. The main advantages of second generation microalgal systems are that they: (1) Have a higher photon conversion efficiency (as evidenced by increased biomass yields per hectare): (2) Can be harvested batch-wise nearly all-year-round, providing a reliable and continuous supply of oil: (3) Can utilize salt and waste water streams, thereby greatly reducing freshwater use: (4) Can couple CO2-neutral fuel production with CO2 sequestration: (5) Produce non-toxic and highly biodegradable biofuels. Current limitations exist mainly in the harvesting process and in the supply of CO2 for high efficiency production. This review provides a brief overview of second generation biodiesel production systems using microalgae.

https://www.springer.com/cda/content/document/cda_downloaddocument/BioEnergyResearch_Second+Generation+Biofuels+High-Efficiency+Microalgae+for+Biodiesel+Production.pdf?SGWID=0-0-45-1347846-0

Second generation biofuels: high-efficiency microalgae for biodiesel production

A possible source of biological material for the production of biodiesel is represented by microalgae, in particular by their lipid content. The aim of the present work was to study of the effects of temperature and nitrogen concentration on the lipid content of Nannochloropsis oculata and Chlorella vulgaris in view of their possible utilization as novel raw materials for biodiesel production. In addition, various lipid extraction methods were investigated. The extracted lipids were quantitatively and qualitatively analyzed by gravimetric and gas chromatographic methods, respectively, in order to check their suitability according to the European standards for biodiesel. The lipid content of microalgae was strongly influenced by the variation of tested parameters; indeed, an increase in temperature from 20 to 25 °C practically doubled the lipid content of N. oculata (from 7.90 to 14.92%), while an increase from 25 to 30 °C brought about a decrease of the lipid content of C. vulgaris from 14.71 to 5.90%. On the other hand, a 75% decrease of the nitrogen concentration in the medium, with respect to the optimal values for growth, increased the lipid fractions of N. oculata from 7.90 to 15.31% and of C. vulgaris from 5.90 to 16.41%, respectively.

Effect of temperature and nitrogen concentration on the growth and lipid content of nannochloropsis oculata and chlorella vulgaris for biodiesel production

https://oatao.univ-toulouse.fr/2771/1/joannis-cassan_2771.pdf

Commercial applications of microalgae

https://hal.archives-ouvertes.fr/hal-01990463/document

Binding of zearalenone, aflatoxin B1, and ochratoxin A by yeast-based products: a method for quantification of adsorption performance.

BACKROUND: Spirulina platensis (Toliara, Madagascar) provides a food supplement which can fight against malnutrition and food insufficiency in Madagascar. In this country, the current production from three open basins does not cover the need and presents drawbacks, such as low productivity and possible contamination. So cultivation of S. platensis in a closed photobioreactor opens the possibility of extending this microalgae production. In this study, the influence of colour and intensity of the light on S. platensis growth and protein content was investigated in a bubble column. 
RESULTS: Growth kinetics were obtained for four colours (green, white, red and blue) and four intensities (400, 800, 1000 and 1200 lux) of light. The influence of light colour on Spirulina growth was discussed. The highest productivity (183.6 mg L-1d-1) and concentration (2643 mg L-1) were obtained for green light at 1200 lux. The protein content was 58 %. 
CONCLUSION: Using green light allows to improve S. platensis growth. The performances obtained with the closed system are higher than those reported in the literature.

Influence of light quality and intensity in the cultivation of Spirulina platensis from Toliara (Madagascar) in a closed system

Optimization of Nannochloropsis oculata growth was undertaken using the Response Surface Method. A central composite design was defined to study the effects of temperature, pH, incident light intensity and aeration rate on the maximum growth rate of the microalga. Using statistical analysis, the first model calculated to fit results was twice improved. The final model obtained was used to clarify the effects of each factor and their interactions on the growth of Nannochloropsis oculata. The optimum growth conditions of this microorganism were also estimated as 21°C, 52 µmol photons.m-2.s-1, pH 8.4 and 14.7 VVH of aeration rate. These conditions were tested and validated experimentally since the maximum growth rate achieved with these parameters, 0.0359h-1, is the best reported in this study.

Claire Joannis-Cassan

Papers

Commercial applications of microalgae

Binding of zearalenone, aflatoxin B1, and ochratoxin A by yeast-based products: a method for quantification of adsorption performance.

Influence of light quality and intensity in the cultivation of Spirulina platensis from Toliara (Madagascar) in a closed system

Optimization of Nannochloropsis oculata growth using the response surface method

Improvement of the growth of Arthrospira (Spirulina) platensis from Toliara (Madagascar): Effect of agitation, salinity and CO2 addition