Cláudio Wageck Canal

Bio: Cláudio Wageck Canal is an academic researcher from Universidade Federal do Rio Grande do Sul. The author has contributed to research in topics: Pestivirus & Phylogenetic tree. The author has an hindex of 27, co-authored 158 publications receiving 2362 citations. Previous affiliations of Cláudio Wageck Canal include University of Caxias do Sul & Universidade Luterana do Brasil.

Evaluation of selective and non-selective enrichment PCR procedures for Salmonella detection.

Abstract: Aims: To compare PCR combined with enrichment media with the standard microbiological techniques (SMT) and to determine the most sensitive method for the detection of Salmonella and the identification of Salm. typhimurium (ST), Salm. enteritidis (SE), Salm. gallinarum (SG) and Salm. pullorum (SP). Methods and Results: We analysed 87 samples from poultry using PCR and SMT, PCR being performed from non-selective (NS) and Rappaport-Vassiliadis (RV) media. PCR-NS was less sensitive than PCR-RV and SMT for the detection and identification of Salmonella. PCR-RV detected more positive samples of Salmonella sp. than SMT but both these methods showed similar sensitivity regarding the identification of Salmonella serovars. Conclusions: PCR-RV was more sensitive and decreased the time necessary to detect and identify Salmonella. Significance and Impact of the Study: PCR-RV is a powerful tool for the rapid and accurate detection and identification of Salmonella and can be implemented in diagnostic and food analysis laboratories.

Tropism and molecular pathogenesis of canine distemper virus.

Abstract: Canine distemper virus (CDV), currently termed Canine morbillivirus, is an extremely contagious disease that affects dogs. It is identified as a multiple cell tropism pathogen, and its host range includes a vast array of species. As a member of Mononegavirales, CDV has a negative, single-stranded RNA genome, which encodes eight proteins. Regarding the molecular pathogenesis, the hemagglutinin protein (H) plays a crucial role both in the antigenic recognition and the viral interaction with SLAM and nectin-4, the host cells’ receptors. These cellular receptors have been studied widely as CDV receptors in vitro in different cellular models. The SLAM receptor is located in lymphoid cells; therefore, the infection of these cells by CDV leads to immunosuppression, the severity of which can lead to variability in the clinical disease with the potential of secondary bacterial infection, up to and including the development of neurological signs in its later stage. Improving the understanding of the CDV molecules implicated in the determination of infection, especially the H protein, can help to enhance the biochemical comprehension of the difference between a wide range of CDV variants, their tropism, and different steps in viral infection. The regions of interaction between the viral proteins and the identified host cell receptors have been elucidated to facilitate this understanding. Hence, this review describes the significant molecular and cellular characteristics of CDV that contribute to viral pathogenesis.

SPAdes, a new genome assembly algorithm and its applications to single-cell sequencing ( 7th Annual SFAF Meeting, 2012)

Abstract: The lion's share of bacteria in various environments cannot be cloned in the laboratory and thus cannot be sequenced using existing technologies. A major goal of single-cell genomics is to complement gene-centric metagenomic data with whole-genome assemblies of uncultivated organisms. Assembly of single-cell data is challenging because of highly non-uniform read coverage as well as elevated levels of sequencing errors and chimeric reads. We describe SPAdes, a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler (specialized for single-cell data) and on popular assemblers Velvet and SoapDeNovo (for multicell data). SPAdes generates single-cell assemblies, providing information about genomes of uncultivatable bacteria that vastly exceeds what may be obtained via traditional metagenomics studies. SPAdes is available online ( http://bioinf.spbau.ru/spades ). It is distributed as open source software.

鳥類の内科学と外科学 : Avian medicine and surgery

Abstract: Section I: The Normal Bird: Systematics. Reproductive Physiology. Nutrition. Laws and Regulations Affecting Aviculture and the Pet Bird Industry. Embryology. Pediatricsand Hand Raising. Behavior. Avicultural Medicine and Flock Health Management. Nonsurgical Methods of Avian Sex Identification. Section II: Diagnostics, Hospital Techniques, And Supportive Care: Physical Examination. Avian Clinical Pathology: Hematologyand Chemistry. Diagnostic Necropsy and Pathology. Radiology. Ultrasonography. Cytology. Differential Diagnostics. Hospital Techniques and Supportive Care. Section III: Infectious Diseases: Bacteriology. Virology. Mycotic Diseases. Parasitology. Zoonotic Diseases. Chlamydia. Mycoplasmal Infections. Section IV: Noninfectious Diseases: Respiratory Disorders. Gastrointestinal Tract. Avian Nervous System. The Endocrine System. Avian Cardiac Disease. Nutritional Disorders. Disorders of the Musculoskeletal System. Avian Dermatology. Disorders of the Special Senses. Neoplasia. Toxicities. Urogenital Disorders. Diseases of the Avian Immune System. Behavioral Problems. Section V: Pharmacology and Therapeutics: Metabolism, Pharmacology, And Therapy. Formulary.