Cosimo Pinto

TL;DR: Following prolonged genotoxic stress, DNA2 and WRN functionally interact to degrade reversed replication forks and promote replication restart, thereby preventing aberrant processing of unresolved replication intermediates.

TL;DR: Evidence is presented that WRN and BLM act epistatically with DNA2 to promote the long-range resection of double strand break ends in human cells and new mechanistic insights are provided into the process of DNA end resection in mammalian cells.

TL;DR: It is shown that the helicase of hDNA2 functionally integrates with BLM or WRN helicases to promote dsDNA degradation by forming a heterodimeric molecular machine, which collectively suggests that the h DNA2 motor promotes the enzyme's capacity to degrade ds DNA in conjunction with BLMor WRN and thus promote the repair of broken DNA.

TL;DR: It is shown that CtIP also dramatically stimulates the adenosine 5′-triphosphate (ATP) hydrolysis-driven motor activity of DNA2 involved in the long-range resection step, which might help explain the less severe resection defects of MRE11 nuclease-deficient cells compared to those lacking CtIP.

TL;DR: It is shown that the motor activity of both recombinant yeast and human DNA2 promotes efficient degradation of long stretches of ssDNA, particularly in the presence of the replication protein A.