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Cristina Lombardo

Bio: Cristina Lombardo is an academic researcher from University of Milan. The author has contributed to research in topics: Ladinian & Actinopterygii. The author has an hindex of 17, co-authored 41 publications receiving 818 citations. Previous affiliations of Cristina Lombardo include National Scientific and Technical Research Council & Facultad de Ciencias Exactas y Naturales.
Topics: Ladinian, Actinopterygii, Genus, Perleidus, Skull roof

Papers
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Journal ArticleDOI
TL;DR: The main discoveries that have emerged at Mar del Plata University over the past 20 years are summarized and discussed, and that have contributed to understand part of the biology of NO in plants.

83 citations

Journal ArticleDOI
TL;DR: The hypothesis that variations in NO levels influence cellulose synthesis and content in roots and three tomato cellulose synthase transcripts were identified and indicated that SICESA1 and SicesA3 levels were affected by high NO concentrations.
Abstract: Nitric oxide (NO) is a bioactive molecule involved in several growth and developmental processes in plants. These processes are mostly characterized by changes in primary and secondary metabolism. Here, the effect of NO on cellulose synthesis in tomato (Solanum lycopersicum) roots was studied. The phenotype of roots, cellulose content, the incorporation of 14C-glucose into cellulosic fraction and the expression of tomato cellulose synthase (CESA) transcripts in roots treated with the NO donor sodium nitroprusside (SNP) were analysed. Nitric oxide affected cellulose content in roots in a dose dependent manner. Low concentrations of SNP (pmoles of NO) increased cellulose content in roots while higher concentrations of SNP (nmoles of NO) had the opposite effect. This result correlated with assays of 14C-glucose incorporation into cellulose in roots. The effect of NO on 14C-glucose incorporation into cellulose was transient and reversible. Microscopic analysis of roots suggested that NO affected primary cell wall cellulose synthesis. Three tomato cellulose synthase (SICESA) transcripts were identified. Reverse transcriptase polymerase chain reaction experiments were carried out and indicated that SICESA1 and SICESA3 levels were affected by high NO concentrations. Together, these results support the hypothesis that variations in NO levels influence cellulose synthesis and content in roots.

77 citations

Journal ArticleDOI
TL;DR: The Italian Caucasian population shows polymorphism of the KIR gene family like other Caucasian and non-Caucasian populations, and although 64 genotypes have been observed, genotype 1 remains the most frequent as already observed in other populations.
Abstract: Killer cell immunoglobulin-like receptors (KIRs) are a family of inhibitory and activatory receptors that are expressed by most natural killer (NK) cells. The KIR gene family is polymorphic: genomic diversity is achieved through differences in gene content and allelic polymorphism. The number of KIR loci has been reported to vary among individuals, resulting in different KIR haplotypes. In this study we report the genotypic structure of KIRs in 217 unrelated healthy Italian individuals from 22 immunogenetics laboratories, located in the northern, central and southern regions of Italy. Two hundred and seventeen DNA samples were studied by a low resolution PCR-SSP kit designed to identify all KIR genes. All 17 KIR genes were observed in the population with different frequencies than other Caucasian and non-Caucasian populations; framework genes KIR3DL3, KIR3DP1, KIR2DL4 and KIR3DL2 were present in all individuals. Sixty-five different profiles were found in this Italian population study. Haplotype A remains the most prevalent and genotype 1, with a frequency of 28.5%, is the most commonly observed in the Italian population. The Italian Caucasian population shows polymorphism of the KIR gene family like other Caucasian and non-Caucasian populations. Although 64 genotypes have been observed, genotype 1 remains the most frequent as already observed in other populations. Such knowledge of the KIR gene distribution in populations is very useful in the study of associations with diseases and in selection of donors for haploidentical bone marrow transplantation.

61 citations

Journal ArticleDOI
TL;DR: Results indicate that both nitric oxide (NO) and flavonoids are systemically induced in UV-B-irradiated maize seedlings and maize leaves expressing the systemic response toUV-B show low cellular damage measured as ion leakage when they are challenged by a second round of irradiation.

60 citations

Journal ArticleDOI
TL;DR: A functional analysis of gamma carbonic anhydrase 2 (CA2) is reported, proposing that reduction in ROS inhibits H2O2 dependent lignin polymerization in CA2 over-expressing plants, thereby causing male sterility.
Abstract: Plant mitochondria include gamma-type carbonic anhydrases (γCAs) of unknown function. In Arabidopsis, the γCAs form a gene family of five members which all are attached to the NADH dehydrogenase complex (complex I) of the respiratory chain. Here we report a functional analysis of gamma carbonic anhydrase 2 (CA2). The gene encoding CA2 is constitutively expressed in all plant organs investigated but it is ten fold induced in flowers, particularly in tapetal tissue. Ectopic expression of CA2 in Arabidopsis causes male sterility in transgenic plants. In normal anther development, secondary thickenings of the endothecial cell wall cause anthers to open upon dehydration. Histological analyses revealed that abnormal secondary thickening prevents anther opening in 35S::CA2 transgenic plants. CA2 abundance in transgenic plants is increased 2–3 fold compared to wild-type plants as revealed by Western blotting analyses. Moreover, abundance of other members of the CA family, termed CA3 and CAL2, is increased in transgenic plants. Oxygen uptake measurements revealed that respiration in transgenic plants is mainly based on NADH reduction by the alternative NADH dehydrogenases present in plant mitochondria. Furthermore, the formation of reactive oxygen species (ROS) is very low in transgenic plants. We propose that reduction in ROS inhibits H2O2 dependent lignin polymerization in CA2 over-expressing plants, thereby causing male sterility.

45 citations


Cited by
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01 Jan 1944
TL;DR: The only previously known species of Myrsidea from bulbuls, M. warwicki ex Ixos philippinus, is redescribed and sixteen new species are described; they and their type hosts are described.
Abstract: We redescribe the only previously known species of Myrsidea from bulbuls, M. pycnonoti Eichler. Sixteen new species are described; they and their type hosts are: M. phillipsi ex Pycnonotus goiavier goiavier (Scopoli), M. gieferi ex P. goiavier suluensis Mearns, M. kulpai ex P. flavescens Blyth, M. finlaysoni ex P. finlaysoni Strickland, M. kathleenae ex P. cafer (L.), M. warwicki ex Ixos philippinus (J. R. Forster), M. mcclurei ex Microscelis amaurotis (Temminck), M. zeylanici ex P. zeylanicus (Gmelin), M. plumosi ex P. plumosus Blyth, M. eutiloti ex P. eutilotus (Jardine and Selby), M. adamsae ex P. urostictus (Salvadori), M. ochracei ex Criniger ochraceus F. Moore, M. borbonici ex Hypsipetes borbonicus (J. R. Forster), M. johnsoni ex P. atriceps (Temminck), M. palmai ex C. ochraceus, and M. claytoni ex P. eutilotus. A key is provided for the identification of these 17 species.

1,756 citations

Journal ArticleDOI
TL;DR: It is difficult to define a bespoke plant NO signalling pathway, but rather NO appears to act as a modifier of other signalling pathways, and early reports that NO signalling involves cGMP-as in animal systems-require revisiting.
Abstract: Background and aims After a series of seminal works during the last decade of the 20th century, nitric oxide (NO) is now firmly placed in the pantheon of plant signals. Nitric oxide acts in plant–microbe interactions, responses to abiotic stress, stomatal regulation and a range of developmental processes. By considering the recent advances in plant NO biology, this review will highlight certain key aspects that require further attention.

433 citations

Journal ArticleDOI
TL;DR: It is shown that antioxidant flavonoids display the greatest capacity to regulate key steps of cell growth and differentiation in eukaryotes and are of great value in photoprotection.

425 citations

Journal ArticleDOI
22 Sep 2011-Blood
TL;DR: Filtration of purified NK cells is feasible in elderly patients with high-risk acute myeloid leukemia, and donor NK cells were shown in vivo by the detection of donor-derived NK clones that killed recipient's targets.

360 citations

Journal ArticleDOI
TL;DR: The issues of enzymatic and chemical generation of NO in plants, NO sensing and downstream signaling, namely the putative cGMP and Ca(2+) pathways, ion-channel activity modulation, gene expression regulation, and the interface with other ROS, which can have a profound effect on both NO accumulation and function are addressed.

358 citations