D
D.A. Korzhenevskiy
Researcher at Kurchatov Institute
Publications - 24
Citations - 251
D.A. Korzhenevskiy is an academic researcher from Kurchatov Institute. The author has contributed to research in topics: HU Protein & Aminoglycoside. The author has an hindex of 7, co-authored 22 publications receiving 153 citations.
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Journal ArticleDOI
Comparison of histone-like HU protein DNA-binding properties and HU/IHF protein sequence alignment
Dmitri E. Kamashev,Yulia К. Agapova,Sergey M. Rastorguev,A. A. Talyzina,Konstantin M. Boyko,Konstantin M. Boyko,D.A. Korzhenevskiy,Anna V. Vlaskina,R. G. Vasilov,Vladimir I. Timofeev,Tatiana V. Rakitina +10 more
TL;DR: Detailed analysis of the similarity and variability of DNA-recognizing and bending of four HU proteins from closely and distantly related HU clades shows that HU fold remains similar in spite of the HU sequence variations.
Journal ArticleDOI
Novel Genetically Encoded Bright Positive Calcium Indicator NCaMP7 Based on the mNeonGreen Fluorescent Protein
Oksana M. Subach,Vladimir P. Sotskov,Viktor V. Plusnin,Anna M. Gruzdeva,Anna M. Gruzdeva,Natalia V. Barykina,Olga I. Ivashkina,Olga I. Ivashkina,Konstantin V. Anokhin,Alena Yu. Nikolaeva,D.A. Korzhenevskiy,Anna V. Vlaskina,Vladimir A. Lazarenko,Konstantin M. Boyko,Tatiana V. Rakitina,Anna M. Varizhuk,Galina E. Pozmogova,Oleg V. Podgorny,Kiryl D. Piatkevich,Kiryl D. Piatkevich,Edward S. Boyden,Fedor V. Subach +21 more
TL;DR: In this article, a green GECI called NCaMP7 was developed for visualization of grating-evoked and place-dependent neuronal activity in the visual cortex and the hippocampus of mice using a two-photon microscope and an NVista miniscope.
Journal ArticleDOI
Structural basis of the high thermal stability of the histone-like HU protein from the mollicute Spiroplasma melliferum KC3
K.M. Boyko,Tatiana V. Rakitina,Tatiana V. Rakitina,D.A. Korzhenevskiy,Anna V. Vlaskina,Yuliya K. Agapova,Dmitry E. Kamashev,Sergey Yu. Kleymenov,Vladimir Popov,Vladimir Popov +9 more
TL;DR: A comparative analysis of the melting curves of mutant and wild-type HUSpm revealed the motifs that play a key role in protein thermal stability: non-conserved phenylalanine residues in the hydrophobic core, an additional hydrophilic loop at the N-terminal region of the protein, and the absence of the internal cavity present at the dimer interface of some HU proteins.
Novel Genetically Encoded Bright Positive Calcium Indicator NCaMP7 Based on the mNeonGreen Fluorescent Protein
Oksana M. Subach,Vladimir P. Sotskov,Viktor V. Plusnin,Anna M. Gruzdeva,Anna M. Gruzdeva,Natalia V. Barykina,Olga I. Ivashkina,Olga I. Ivashkina,Konstantin V. Anokhin,Alena Yu. Nikolaeva,D.A. Korzhenevskiy,Anna V. Vlaskina,Vladimir A. Lazarenko,Konstantin M. Boyko,Tatiana V. Rakitina,Anna M. Varizhuk,Galina E. Pozmogova,Oleg V. Podgorny,Kiryl D. Piatkevich,Kiryl D. Piatkevich,Edward S. Boyden,Fedor V. Subach +21 more
TL;DR: The NCaMP7 indicator was successfully utilized for in vivo visualization of grating-evoked and place-dependent neuronal activity in the visual cortex and the hippocampus of mice using a two-photon microscope and an NVista miniscope, respectively.
Journal ArticleDOI
Expression, purification, crystallization and preliminary X‐ray crystallographic analysis of the histone‐like HU protein from Spiroplasma melliferum KC3
K.M. Boyko,M.A. Gorbacheva,Tatiana V. Rakitina,D.A. Korzhenevskiy,Anna Vanyushkina,Dmitry Kamashev,A.V. Lipkin,Vladimir Popov +7 more
TL;DR: The HU protein from the parasitic mycoplasma Spiroplasma melliferum KC3 was cloned, overexpressed in Escherichia coli and purified to homogeneity and the structure was solved by the molecular-replacement method with one monomer per asymmetric unit.