D. Lo Giudice

Bio: D. Lo Giudice is an academic researcher from University of Messina. The author has contributed to research in topics: Vaccination & Vaccination policy. The author has an hindex of 4, co-authored 5 publications receiving 58 citations.

Hepatitis E virus (HEV) in sewage from treatment plants of Messina University Hospital and of Messina City Council.

Abstract: Samples of sewage from treatment plants at the "G. Martino" University Hospital of Messina (AOU) and that of Messina City Council were analysed to detect the hepatits E virus. Samples were taken on sewage entering and exiting the treatment plants on a monthly basis over a one-year period from both the hospital plant (24 samples) and the municipal plant (22 samples). All sewage samples were pretreated by ultrafiltraton and concentration and finally processed by the PCR method to amplify gene material. A total of three samples tested positive: two (8.33%) entering the AOU treatment plant and one (4.5%) entering the municipal plant while no cases of HEV were detected in samples of treated sewage. These findings confirm the presence of the virus in the city of Messina and showed that the two treatment plants to be working efficiently when tested.

Eliminating congenital rubella: a seroepidemiological study on women of childbearing age and MMR vaccine coverage in newborns.

Abstract: Introduction. Rubella can have particularly serious effects on the product of conception if contracted during pregnancy. Thus, the main aim of rubella vaccination programmes is to prevent infection during pregnancy. Materials and methods. A seroepidemiological study was conducted from July 2006 to December 2007 on 1,000 women of childbearing age, 15 to 45 years old, using specific rubivirus antibody assays, IgG and IgM. A questionnaire administered at the same time allowed us to survey how much women knew about this disease. In addition, MMR vaccine coverage rates were analysed for cohorts born in the local health districts of Messina for the period 1993-2006. Results. An analysis of the replies given to the questionnaire showed an estimated 42.8% of the women to have immunity from rubella, while the serological study showed an immunity coverage rate of 80.6%. Vaccination coverage in the local health districts regarding the first dose of MMR was 81% (cohorts 1993-2005), while the rate was only 24% for the second dose (cohorts 1993-2002). Conclusions. Both immunity coverage in women of childbearing age and that for newborns (for the cohort considered) fall below the 95% target set by the National Elimination Plan for Measles and Congenital Rubella (PNEM). It is therefore necessary to provide women with adequate information about the risks of rubella during pregnancy and about the benefits of vaccination, as well as to recoup subjects at risk or those whose immune status is unknown. Public health authorities also need to make continued efforts to increase the number of MMR vaccinations throughout the region.

Mediterranean diet: knowledge and adherence in Italian young people.

Abstract: INTRODUCTION: In November 2010 the Mediterranean Diet was recognized by the UNESCO as Intangible Cultural Heritage of Humanity. It is an heritage that brings together the dietary habits of the peoples of the Mediterranean countries, consolidated over the centuries remained almost unchanged until the 1950s. Numerous scientific studies have also shown that the Mediterranean Diet is an healthy diet that helps to prevent the main chronic diseases such as cardiovascular diseases, diabetes, bulimia and obesity and thanks to the antioxidant power of olive oil combined with vegetables consumption, an important means of cancer prevention. The aim of our study was to investigate the knowledge and the adherence to the Mediterranean diet of young people living in the Mediterranean area. MATERIALS AND METHODS: The survey was carried out by administering a web-based anonymous questionnaire from March to May 2019 to Italian young people. In this survey we asked for socio-economic conditions, knowledge, awareness and adherence to the Mediterranean diet (through the KIDMED test) both in childhood and at present time. Parental educational status and family income were used as indicators of socio-economic status (SES). RESULT: The sample was made up by 507 Italian young people between the ages of 17 and 35 (123 males and 384 females), with an average age of 22.88 years (DS 7.02). Only 11.4% knew the Mediterranean diet. During childhood 70.8% of respondents had breakfast and 58% ate fruit and vegetables daily. Today they have maintained the correct eating habits, in fact, 71.2% had breakfast, 60.2% ate fruit and 64.7% ate vegetables. In addition, only 48.3% eat fish and the majority of respondents consume carbohydrates (76.9%). To a lesser extent they consume junk foods like candy and various snacks (18.1%) or fast food meals (9.1%). Moreover, we asked for physical activity and we found that 48.9% did it. We found age, exercise and sex high significant variables (p<0.01). Higher adherence to MD was found in older interviewees. CONCLUSION: Our study we found high knowledge to a Mediterranean diet into 90.5% of the sample and poor adherence into 23.5% of them, medium into 53.4% while higher only in 23.1% of the interviewees. Lower adherence was found among those who do not engage in physical activity and the two regressor with higher weight on the adherence to MD were "eat at home "and "eat fruit and vegetables". Furthermore, in our sample more increased the age more increased adherence to traditional diet models.

Seroprevalence of hev antibodies in a sample of pregnant women in the city of Messina.

Abstract: Background Hepatitis E virus (HEV) is widespread in developing countries and the disease is also increasing in the developed ones. This infection in pregnancy can cause spontaneous abortion and neonatal death in 56% of newborns. Study design The study was conducted on a sample of 352 pregnant women, 326 Italian and 26 foreign, in order to confirm the presence of HEV in our territory, to analyze wrong habits of the population and to suggest preventive actions against the risk to contract the infection during pregnancy. Methods We asked all women under study to fill an anonymous questionnaire immediately before taking a venous blood samples to determine the presence of anti-HEV antibodies. The questionnaire contained a set of questions to gain information about lifestyles and risk factors to contract HEV. Results The questionnaire revealed that a portion of the tested women have risk behaviours, as consumption of raw or undercooked food, eating unwashed food and traveling to endemic areas. The percentage of women positive for HEV antibodies was 3.4%, in agreement with national data; all the women were Italian. Conclusions This study confirms the circulation of HEV in the city of Messina. For this reason, it is highly recommended to disseminate hygienic and appropriate behaviours and feeding habits in order to prevent the risk to contract the infection.

Emerging and potentially emerging viruses in water environments

Abstract: Among microorganisms, viruses are best fit to become emerging pathogens since they are able to adapt not only by mutation but also through recombination and reassortment and can thus become able to infect new hosts and to adjust to new environments. Enteric viruses are among the commonest and most hazardous waterborne pathogens, causing both sporadic and outbreak-related illness. The main health effect associated with enteric viruses is gastrointestinal illness, but they can also cause respiratory symptoms, conjunctivitis, hepatitis, central nervous system infections, and chronic diseases. Non-enteric viruses, such as respiratory and epitheliotrophic viruses are not considered waterborne, as they are not readily transmitted to water sources from infected individuals. The present review will focus on viral pathogens shown to be transmitted through water. It will also provide an overview of viruses that had not been a concern for waterborne transmission in the past, but that may represent potentially emerging waterborne pathogens due to their occurrence and persistence in water environments.

Vaccine hesitancy: An overview on parents' opinions about vaccination and possible reasons of vaccine refusal

Abstract: Background. Vaccine hesitancy has increased worldwide with a subsequent decreasing of vaccination rates and outbreaks of vaccine-preventable diseases (i.e. measles, poliomyelitis and pertussis) in several developed countries, including Italy. Design and Methods. We conducted a survey to investigate the attitudes of a parents' sample about vaccinations by the distribution of questionnaires in six lower secondary schools of the Italian city of Messina. Results. Regarding vaccinations carried out on children, the declared vaccination coverage rates ranged widely between good coverage percentages for some vaccinations (Measles-Mumps-Rubella, Diphtheria-Tetanus-Pertussis), and very low coverage rates for others, especially for "new" vaccinations (HPV, meningococcal, pneumococcal). The vaccinations carried out correlated negatively with both parents' age and their level of education. Moreover, a favourable parents' opinion was strongly influenced by a favourable opinion of the physician, while an unfavourable parents' opinion seemed conditioned by a direct or indirect knowledge of people harmed by vaccines. In addition, our data show that parents do not often know or partially know the real composition of the vaccines and the diseases prevented by vaccinations. Conclusions. Data analysis shows that parents are, theoretically, favourable towards vaccinations but have little knowledge of such practices, sometimes not being unaware of the types of vaccines administrated to their children. Health education and communication of correct information are certainly the cornerstones to improve the situation and to fight the widespread and non-grounded fears about vaccines.

The epidemiology of hepatitis E virus infections in developed countries and among immunocompromised patients

Abstract: Hepatitis E virus (HEV) is an important cause of acute hepatitis in humans worldwide, both as epidemic and sporadic disease Since the virus was identified in 1983, epidemics have occurred regularly in many countries across South and Southeast Asia when seasonal floods have contaminated drinking water supplies and in Africa during humanitarian crises among refugee populations without access to clean water In addition, sporadic cases and small clusters of HEV infections have been recognized throughout the world in developed countries over the past couple of decades This review will focus on emerging evidence of HEV infection as an under-recognized pathogen in Europe, the USA and other industrialized countries We will discuss some of the issues associated with the recognition, diagnosis and treatment of these sporadic cases We will also summarize the recent literature on autochthonous HEV infection among populations in developed countries in industrialized Europe, the USA, Japan and other industrialized Asian countries We will review recent reports of acute and chronic HEV infections among transplant recipients and other immunocompromised individuals including HIV/AIDS patients

Hepatitis E Virus in Industrialized Countries: The Silent Threat.

Abstract: Hepatitis E virus (HEV) is the main cause of acute viral hepatitis worldwide. Its presence in developing countries has been documented for decades. Developed countries were supposed to be virus-free and initially only imported cases were detected in those areas. However, sporadic and autochthonous cases of HEV infection have been identified and studies reveal that the virus is worldwide spread. Chronic hepatitis and multiple extrahepatic manifestations have also been associated with HEV. We review the data from European countries, where human, animal, and environmental data have been collected since the 90s. In Europe, autochthonous HEV strains were first detected in the late 90s and early 2000s. Since then, serological data have shown that the virus infects quite frequently the European population and that some species, such as pigs, wild boars, and deer, are reservoirs. HEV strains can be isolated from environmental samples and reach the food chain, as shown by the detection of the virus in mussels and in contaminated pork products as sausages or meat. All these data highlight the need of studies directed to control the sources of HEV to protect immunocompromised individuals that seem the weakest link of the HEV epidemiology in industrialized regions.