Author
Daisuke Nakada
Other affiliations: Columbia University
Bio: Daisuke Nakada is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Messenger RNA & Protein biosynthesis. The author has an hindex of 5, co-authored 6 publications receiving 343 citations. Previous affiliations of Daisuke Nakada include Columbia University.
Papers
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TL;DR: During the induction phase a messenger RNA specific for β-galactosidase is produced which directs the subsequent synthesis of the enzyme in the inducer-free medium, which largely prevents the subsequent production of normal enzyme and causes the formation of an altered protein serologically related to β-Galactosids.
260 citations
34 citations
TL;DR: Escherichia coli grown in succinate medium is starved of energy when deprived of oxygen and under these anaerobic conditions β -galactosidase-specific messenger RNA can be partially protected from decay in functional form.
27 citations
TL;DR: It was found that under these conditions of starvation neither synthesis of new ribosomes nor turnover of ribosomal RNA and protein occurs, and on the other hand, the turnover of messenger RNA continues during uracil starvation.
10 citations
TL;DR: It is concluded that pre-existing ribosomes can make a new species of bacterial protein.
10 citations
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TL;DR: The action of cyclic AMP in E. coli may serve as a model to understand its action on transcriptional and translational processes in eukaryotes.
Abstract: Both cyclic AMP and a specific inducer acting in concert are required for the synthesis of many inducible enzymes in E. coli. Little enzyme is made in the absence of either. In contrast to the specific inducers which stimulate the synthesis only of the proteins required for their metabolism, cyclic AMP controls the synthesis of many proteins. Glucose and certain other carbohydrates decrease the differential rate of synthesis of inducible enzymes by lowering cyclic AMP concentrations. In the lac operon, cyclic AMP acts at the promoter site to facilitate initiation of transcription. This action requires another protein, the cyclic AMP receptor protein. The nucleotide stimulates tryptophanase synthesis at a translational level. The action of cyclic AMP in E. coli may serve as a model to understand its action on transcriptional and translational processes in eukaryotes.
472 citations
TL;DR: This is a review on the mechanism of action of FUra, where three main areas are addressed: metabolism, RNA-directed actions of F Ura, and DNA- directed actions ofFUra.
425 citations
TL;DR: A review of the major pharmacological and biochemical effects of the fluorinated pyrimidine nucleosides can be found in this paper, where the authors focus mainly on key references that are germane to the topics being described.
Abstract: Since their introduction (Heidelberger et al., 1957), the fluorinated pyrimidines and their nucleosides have been widely used as biochemical tools for the elucidation of a number of problems encountered in cell biology and molecular biology. Of more practical importance, however, has been their extensive use as drugs for the palliative treatment of patients suffering from disseminated cancer. One of the compounds is now clinically useful in the curative treatment of herpes simplex viral infections of the eye. Although these compounds have generated an enormous literature, this chapter will deal primarily with a review of the major pharmacological and biochemical effects of these compounds; the coverage of the literature, by necessity, will focus only on key references that are germane to the topics being described. Several reviews of this topic have appeared elsewhere (Heidelberger and Ansfield, 1963; Heidelberger, 1965, 1966, 1967, 1969, 1970, 1973; Mandel, 1969; Carter, 1970).
367 citations
TL;DR: The role of cyclic AMP in regulation of gene expression and its possible role as a negative element in CRP structure are explained.
Abstract: INTRODUCTION ................ ROLE OF CYCLIC AMP IN REGULATION OF GENE EXPRESSION .......... A Positive Element in Gene Expression ...................................... CRP .......................... :........................................... Direct stimulation of gene expression ...................................... Possible Role As a Negative Element ......................................... Does Cyclic AMP Rave Other Actions in E. co .............................. OPERON ACTIVATION BY CYCLIC AMP .................................... Genetic analysis of the lac promoter ....................................... CONTROL OF CYCLIC AMP LEVELS ........................................ Adenylate Cyclase ........................................................... Cyclic AMP Phosphodiesterase ...................................... Cyclic AMP Release ......................................................... Rate of Cyclic AMP Synthesis ............................................... CYCLIC AMP RECEPTOR PROTEIN ........................................ Physical properties of CRP ................................................ Cyclic AMP binding .... Promoter-specific DNAbinding. Effects of cyclic AMP on CRP structure .................................... CYCLIC AMP AND BACTERIOPHAGE A ...................................... CYCLIC GMP ................................................................ CONCLUSION ................................................................ LITERATURE CITED ........................................................
333 citations
TL;DR: The administration of actinomycin D or 5-fluorouracil to rats previously treated with allylisopropylacetamide results in a rapid decline of the hepatic levels of induced δ-aminolevulinic acid synthetase, which suggests that the turnover of the messenger RNA for hepatic δ, £2,000-3,000 acids is considerably more rapid than the bulk of rat liver messenger RNA.
265 citations