D
Dane R. Roberts
Researcher at BC Research Inc
Publications - 18
Citations - 1012
Dane R. Roberts is an academic researcher from BC Research Inc. The author has contributed to research in topics: Somatic embryogenesis & Storage protein. The author has an hindex of 15, co-authored 18 publications receiving 998 citations.
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Synchronous and high frequency germination of interior spruce somatic embryos following partial drying at high relative humidity
TL;DR: Drying over a range of humidities indicated that humidities of 81% and lower were lethal to the embryos, whereas germination was enhanced following treatment at humidities greater than 95% relative to untreated controls.
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Abscisic acid and indole‐3‐butyric acid regulation of maturation and accumulation of storage proteins in somatic embryos of interior spruce
TL;DR: Root elongation, which was used as a measure of embryo quality, was never observed from shooty embryo Structures and was 2-3 fold higher in mature embryos compared to those that germinated precociously.
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Propagation of interior spruce by somatic embryogenesis.
TL;DR: Production of mature somatic embryos was highest at 40 and 60 μM abscisic acid, while treatment of mature embryos with a high relative humidity treatment resulted in partial drying of the embryos and upon rehydration, markedly enhan...
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Abscisic acid and mannitol promote early development, maturation and storage protein accumulation in somatic embryos of interior spruce
TL;DR: Pre-treatment of late cotyledonary embryos with mannitol did not increase the frequency of germination compared with germination in non-treated embryos although dehydration with high relative humidity treatment increased germination to 83%.
Journal ArticleDOI
Cryopreservation of interior spruce (Picea glauca engelmanni complex) embryogenic cultures.
David R. Cyr,Wayne R. Lazaroff,Susanna M. A. Grimes,Grant Quan,Terry D. Bethune,David I. Dunstan,Dane R. Roberts +6 more
TL;DR: The results of this work indicate the applicability of cryopreservation as a long-term storage strategy for spruce embryogenic cultures from a wide genetic background.