D
Daniel R. Perez
Researcher at University of Georgia
Publications - 222
Citations - 13587
Daniel R. Perez is an academic researcher from University of Georgia. The author has contributed to research in topics: Virus & Influenza A virus. The author has an hindex of 55, co-authored 198 publications receiving 12208 citations. Previous affiliations of Daniel R. Perez include Crips & Virginia–Maryland Regional College of Veterinary Medicine.
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Universal primer set for the full-length amplification of all influenza A viruses.
TL;DR: The resultant primer set is suitable for all influenza A viruses to generate full-length cDNAs, to subtype viruses, to sequence their DNA, and to construct expression plasmids for reverse genetics systems.
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Generation of influenza A viruses entirely from cloned cDNAs
Gabriele Neumann,Tokiko Watanabe,Hiroshi Ito,Shinji Watanabe,Hideo Goto,Peng Gao,Mark A. Hughes,Daniel R. Perez,Ruben O. Donis,Erich Hoffmann,Gerd Hobom,Yoshihiro Kawaoka +11 more
TL;DR: A new reverse-genetics system that allows one to efficiently generate influenza A viruses entirely from cloned cDNAs is described, which should be useful in viral mutagenesis studies and in the production of vaccines and gene therapy vectors.
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Eight-plasmid system for rapid generation of influenza virus vaccines.
Erich Hoffmann,Scott Krauss,Daniel R. Perez,Richard J. Webby,Robert G. Webster,Robert G. Webster +5 more
TL;DR: These findings demonstrate that the eight-plasmid system allows the rapid and reproducible generation of reassortant influenza A viruses for use in the manufacture of vaccines.
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A new influenza virus virulence determinant: The NS1 protein four C-terminal residues modulate pathogenicity
TL;DR: Viruses containing NS1 sequences from the 1918 H1N1 and H5N1 highly pathogenic avian influenza (HPAI) viruses demonstrated increased virulence in infected mice compared with wt A/WSN/33 virus, suggesting the NS1 protein C terminus may interact with PDZ-binding protein(s) and modulate pathogenicity through alternative mechanisms.
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Distinct contributions of vaccine-induced immunoglobulin G1 (IgG1) and IgG2a antibodies to protective immunity against influenza.
Victor C. Huber,Raelene M. McKeon,Martha N. Brackin,Laura Miller,Rachael Keating,Scott A. Brown,Natalia Makarova,Daniel R. Perez,Gene H. MacDonald,Jonathan A. McCullers +9 more
TL;DR: Two separate but important roles for both IgG1 and IgG2a expression in vaccination against influenza are detailed and it is argued for the development of vaccine regimens that stimulate and measure expression of both antibody isotypes.