Author
Daokun Guan
Bio: Daokun Guan is an academic researcher from Yantai University. The author has contributed to research in topics: Multiple drug resistance & Paclitaxel. The author has an hindex of 2, co-authored 2 publications receiving 66 citations.
Topics: Multiple drug resistance, Paclitaxel, P-glycoprotein
Papers
More filters
TL;DR: H6 is a novel and potent MDR reversal agent, which has the potential to be administered in combination with conventional anticancer drugs and demonstrates robust reversal activity against MDR in vitro and in vivo.
75 citations
Patent•
14 Dec 2016
TL;DR: In this paper, the alpha-helexin derivatives of triterpene saponin derivatives were described and a pharmaceutical composition containing the derivatives, and application of the derivatives in resisting neoplastic diseases.
Abstract: The invention relates to the fields of organic synthesis and pharmaceutical chemistry, and particularly discloses novel-structure alpha-helexin derivatives The invention also discloses a preparation method of the triterpene saponin derivatives, a pharmaceutical composition containing the derivatives, and application of the derivatives in resisting neoplastic diseases
4 citations
Cited by
More filters
TL;DR: This review presents up‐to‐date information on the structural and functional aspects of P‐glycoprotein and its known inhibitors and provides some information on drug discovery approaches for candidate P‐gp inhibitors.
102 citations
25 Jul 2019
TL;DR: Results indicated that CBD could induce G0–G1 phase cell cycle arrest and apoptosis by increasing ROS production, leading to the inhibition of SGC-7901 cell proliferation, thereby suggesting that CBD may have therapeutic effects on gastric cancer.
Abstract: The main chemical component of cannabis, cannabidiol (CBD), has been shown to have antitumor properties. The present study examined the in vitro effects of CBD on human gastric cancer SGC-7901 cells. We found that CBD significantly inhibited the proliferation and colony formation of SGC-7901 cells. Further investigation showed that CBD significantly upregulated ataxia telangiectasia-mutated gene (ATM) and p53 protein expression and downregulated p21 protein expression in SGC-7901 cells, which subsequently inhibited the levels of CDK2 and cyclin E, thereby resulting in cell cycle arrest at the G0–G1 phase. In addition, CBD significantly increased Bax expression levels, decreased Bcl-2 expression levels and mitochondrial membrane potential, and then upregulated the levels of cleaved caspase-3 and cleaved caspase-9, thereby inducing apoptosis in SGC-7901 cells. Finally, we found that intracellular reactive oxygen species (ROS) increased after CBD treatment. These results indicated that CBD could induce G0–G1 phase cell cycle arrest and apoptosis by increasing ROS production, leading to the inhibition of SGC-7901 cell proliferation, thereby suggesting that CBD may have therapeutic effects on gastric cancer.
64 citations
TL;DR: PLQ/GA nanoparticles provide a synergistic strategy for effective targeted co-delivery of chemotherapeutic and antiangiogenic agents and reversing MDR and metastasis in breast cancer.
60 citations
TL;DR: It is shown that multiple MiT-TFE transcription factors including TFEB, TFE3 and MITF, which are master regulators of autophagy and lysosomal biogenesis, can be activated upon direct cysteine oxidation by ROS.
Abstract: Significant evidences indicate that reactive oxygen species (ROS) can induce macroautophagy/autophagy under both physiological and pathological conditions. Although the relationship between ROS and...
52 citations
TL;DR: Withaferin A is a bioactive compound with a remarkable anti‐cancer effect derived from Withania somnifera, commonly known as ashwagandha, but the anti-cancer mechanisms of WA in glioblastoma multiforme (GBM) are still unclear.
Abstract: Objective Withaferin A (WA) is a bioactive compound with a remarkable anti-cancer effect derived from Withania somnifera, commonly known as ashwagandha. However, the anti-cancer mechanisms of WA in glioblastoma multiforme (GBM) are still unclear. Materials and methods Cell viability assays and xenografted nude mice were used to evaluate the effects of WA, along with flow cytometry to detect apoptosis and cell cycle of GBM. RNA-seq analysis, Western blotting, immunofluorescence staining, qRT-PCR and siRNA gene silencing were carried out to determine the signalling pathways affected by WA. Results Withaferin A significantly inhibited the growth of GBM in vitro and in vivo and triggered the intrinsic apoptosis of GBM cells by up-regulating expression of Bim and Bad. WA arrested GBM cells at the G2/M phase of the cell cycle through dephosphorylating Thr161 of CDK1 by activating p53-independent p21 up-regulation. Knockdown of p21 restored cell cycle progression and cell viability by down-regulating the expression of Bad rather than Bim. We demonstrated that endoplasmic reticulum (ER) stress induced by WA through the ATF4-ATF3-CHOP axis, initiated apoptosis and G2/M arrest in GBM cells. Conclusion We revealed a novel pathway that elucidated WA activation of apoptosis and G2/M arrest in GBM cells through the ATF4-ATF3-CHOP axis. This discovery is important for optimization of WA-based regimens for prevention and/or treatment of GBM.
46 citations