Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction

Coffee Pulp: An Industrial By-product with Uses in Agriculture, Nutrition and Biotechnology

Caffeine: A potential strategy to improve survival of neonatal pigs and sheep.

Aim: The purpose of this study was to seek the acute effects of coffee intake on glucose metabolism and some hormones
Materials and Methods: The participants were asked to drink a cup of instant coffee (2 g) Three hours later, the glucose, insulin, glucagon, leptin, cortisol, total cholesterol, triglyceride, HDL, LDL, CRP, TSH and free T4 tests were repeated The participants did not consume any other drink or food between 0 and 3 h The participants were called back one week later In 0 h, the glucose, insulin, glucagon, leptin, cortisol, total cholesterol, triglyceride, HDL, LDL, CRP, TSH and free T4 tests were performed This time, the participants were asked to drink a glass of hot water Three hours later, same tests were repeated
Results: There weren't any significant changes in the levels of insulin, glucagon, cortisol, lipid levels, CRP, TSH and free T4 tests after coffee intake but we found significant changes in the leptin and plasma glucose values
Conclusions: In conclusion, acute coffee intake does not have an acute impact on glucagon, cortisol, and TSH levels but affects leptin levels which can be one of the mechanisms of type diabetes mellitus reduction with coffee intake

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The acute effect of coffee intake on hormones that affect glucose and glucose metabolism in healthy individuals

Background: Coffee pulp has a high content of antioxidants capable of modifying the oxidative status in small ruminants. However, high amounts for a prolonged time can reduce fertility. Objective: To determine the effect of two inclusion levels of coffee pulp during estrous synchronization on reproductive variables and oxidative status of primiparous ewes. Methods: Sixty Suffolk x Dorset primiparous ewes were distributed into three treatments in a completely randomized design; T0: (n=20) 1.5 kg balanced diet, Tl: (n=20) 1.5 kg balanced diet and 5% coffee pulp, T2: (n=20) 1.5 kg balanced diet and 10% coffee pulp. Supplementation was given for 16 days before estrus synchronization and until the beginning of the breeding season. A progestogen (CIDR®) was inserted for ll days and a dose of PGF2a was applied two days prior to its withdrawal. Estrus detection started 12 hours after CIDR withdrawal. Blood samples were obtained during the supplementation period to measure oxidative status, antioxidant capacity, glucose and insulin, and up to 9 days after breeding to determine progesterone concentration. Pregnancy diagnosis was performed at 30 and 60 days post-breeding. An analysis of repeated measures of mixed effects and frequency analysis were carried out. Results: Inclusion of coffee pulp for a short period prior to breeding did not affect reproductive parameters, nor progesterone, glucose or insulin concentrations (p>0.05); however, antioxidant capacity increased, while lipid oxidation showed an opposite trend (p<0.05). Conclusion: Inclusion of up to 10% coffee pulp in the diet of ewe lambs for 16 days prior to breeding improves oxidative status without causing adverse effects on pregnancy, estrus or prolificacy.

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David A Contreras-Caro-Del-Castillo

Papers

Coffee pulp supplementation prior to breeding improves oxidative status without affecting fertility of primiparous ewes