Author
David B. Weinstein
Bio: David B. Weinstein is an academic researcher from University of Pennsylvania. The author has contributed to research in topics: Cell membrane & Membrane. The author has an hindex of 4, co-authored 4 publications receiving 1245 citations.
Topics: Cell membrane, Membrane, Membrane lipids, Membrane protein, Glycolipid
Papers
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TL;DR: A rapid method is described for charring 5-300 micrograms of lipids (with concentrated sulfuric acid in a test tube) and estimating them with a reproducibility of +/-1%.
1,037 citations
TL;DR: In this article, four glycolipid classes were isolated from mouse fibroblasts (L cells) and accounted for 0.7% of the total cell lipid, including short chain saturated fatty acids and disialoganglioside.
123 citations
TL;DR: The lipid compositions of L cell and liver cell surface membranes are basically similar and may be useful in classifying surface membrane preparations, according to the comparison reported in the literature.
111 citations
TL;DR: When BHK-21 cells are pulse-labeled with (2-3H)-glycerol for 1 hr, followed by chase periods of 0–22 hrs, rapid turnover of the newly synthesized major phospholipids is observed 2–6 hrs after termination of the pulse, indicating a half-life of 2.0–2.5 hrs.
22 citations
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TL;DR: The experiments showed that the eustigmatophyte Nannochloropsis sp.
Abstract: Thirty microalgal strains were screened in the laboratory for their biomass productivity and lipid content. Four strains (two marine and two freshwater), selected because robust, highly productive and with a relatively high lipid content, were cultivated under nitrogen deprivation in 0.6-L bubbled tubes. Only the two marine microalgae accumulated lipid under such conditions. One of them, the eustigmatophyte Nannochloropsis sp. FM102: 100–112. © 2008 Wiley Periodicals, Inc.
2,714 citations
TL;DR: The majority of neutral glycolipids present in plasma membranes are cryptic, and further extensive studies of the organization of glycolIPid in other eukaryotic cell membranes are necessary.
Abstract: The idea that glycosphingolipids (or, briefly, glycolipids) are ubiquitous components of plasma membrane and display cell type-specific patterns perhaps stemmed from the classical studies on glycolipids of erythrocyte membranes.(1,2) Subsequently, plasma membranes of various animal cells were successfully isolated and analyzed; all were characterized by their much higher content of glycolipid than was found in intracellular membranes.(3–8) It is generally assumed that glycolipids are present at the outer leaflet of the plasma membrane bilayer, although this assumption is based only on experiments with surface-labeling by galactose oxidase-NaB[3H]4 of intact and lysed erythrocyte membranes and inside-out vesicles.(9,10) Obviously, further extensive studies of the organization of glycolipid in other eukaryotic cell membranes are necessary. Interestingly, the majority of neutral glycolipids present in plasma membranes are cryptic (see Section 4.2.1).
1,637 citations
TL;DR: Membrane fatty acid composition, phospholipid composition, and cholesterol content can be modified in many different kinds of intact mammalian cells, and many of the functional responses probably are caused directly by the membrane lipid structural changes, which affect either bulk lipid fluidity or specific lipid domains.
1,437 citations
TL;DR: Treatment of erythrocytes with galactose oxidase followed by reduction with tritiated sodium borohydride at pH 7.4 allowed the labeling of galactosyl and N-acetylgalactosaminyl residues on external surfaces of cells with tritium.
662 citations
TL;DR: The feeding and metabolic rates of Mytilus edulis L.Edulis of different body sizes were measured in response to changes in particle concentrations ranging from 2 to 350 mg l-1, and the particulate carbohydrate, protein and lipid content provided an estimate of the food content of the seston.
Abstract: The feeding and metabolic rates of Mytilus edulis L. of different body sizes were measured in response to changes in particle concentrations ranging from 2 to 350 mg l-1. Rates of oxygen consumption were not significantly affected by changes in seston concentration, whereas clearance rates gradually declined with increasing particle concentration. Pseudofaeces production was initiated at relatively low seston concentrations (<5 mg l-1). Marked seasonal changes were recorded in the composition of suspended particulates (seston) in an estuary in south-west England. Total seston was sampled at frequent intervals throughout an annual cycle and analysed in terms of: particle size-frequency distributions, total dry weight (mg l-1), inorganic content, chlorophyll a, carbohydrate, protein and lipid. The particulate carbohydrate, protein and lipid content provided an estimate of the food content of the seston. The results are discussed in terms of the “food available” to a nonselective suspension feeder, such as M. edulis, during a seasonal cycle. The effect of inorganic silt in suspension was mainly to limit by “dilution” the amount of food material ingested rather than to reduce the amount of material filtered by the mussel. In winter, the food content of the material ingested was 5%, and this increased to 25% during the spring and summer.
577 citations