scispace - formally typeset
Search or ask a question

Showing papers by "David Baltimore published in 1969"


Journal ArticleDOI
TL;DR: Observations begin to establish a molecular basis for understanding the ability of T particles to interfere with the growth of B particles in Chinese hamster ovary cells.
Abstract: Plaque-forming B particles of vesicular stomatitis virus (VSV) induce the synthesis of virus-specific ribonucleic acid (RNA) in Chinese hamster ovary cells, whereas defective T particles do not. Infection with low input multiplicities of B results in the formation of four species of RNA. During infection with high multiplicities, RNA synthesis begins with mainly these four species of RNA but gradually shifts to a new pattern of RNA synthesis involving five other species of RNA. The change can also be induced by superinfection with T at 2.5 hr after infection with a low multiplicity of B. T added at the same time as B prevents virtually all RNA synthesis. Synthesis of the first group of RNA species correlates with the formation of B particles, whereas synthesis of the second group correlates with the formation of T particles. The various species of RNA formed after infection with VSV particles include single-stranded RNA, a completely double-stranded RNA, and RNA with partially double-stranded regions. These observations begin to establish a molecular basis for understanding the ability of T particles to interfere with the growth of B particles.

223 citations



01 Jan 1969
TL;DR: Observations begin to establish amolecular basis forunderstanding theability ofT particles tointerfere with thegrowth ofB particles, and RNA synthesis begins with mainly these fourspecies ofRNA butgradually shifts to newpattern ofRNA synthesis involving five other species ofRNA.
Abstract: Plaque-forming Bparticles ofvesicular stomatitis virus (VSV) induce thesynthesis ofvirus-specific ribonucleic acid(RNA)inChinese hamster ovarycells, whereas defective T particles donot.Infection withlowinput multiplicities ofB results in theformation offourspecies ofRNA.During infection withhighmultiplicities, RNA synthesis begins withmainly these fourspecies ofRNA butgradually shifts toanewpattern ofRNA synthesis involving five other species ofRNA.Thechange canalsobeinduced bysuperinfection withT at2.5hrafter infection withalow multiplicity ofB.TaddedatthesametimeasBprevents virtually all RNA synthesis. Synthesis ofthefirst group ofRNA species correlates withtheformation ofBparticles, whereas synthesis ofthesecond groupcorrelates withtheformation ofT particles. Thevarious species ofRNA formedafter infection withVSV particles include single-stranded RNA,acompletely double-stranded RNA,andRNA with partially double-stranded regions. Theseobservations begin toestablish amolecular basis forunderstanding theability ofT particles tointerfere withthegrowth ofB particles.

24 citations