D
David R. Mole
Researcher at University of Oxford
Publications - 75
Citations - 16932
David R. Mole is an academic researcher from University of Oxford. The author has contributed to research in topics: Hypoxia-inducible factors & Transcription factor. The author has an hindex of 40, co-authored 68 publications receiving 15010 citations. Previous affiliations of David R. Mole include John Radcliffe Hospital & University of Edinburgh.
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Journal ArticleDOI
Targeting of HIF-alpha to the von Hippel-Lindau Ubiquitylation Complex by O2-Regulated Prolyl Hydroxylation
Panu Jaakkola,David R. Mole,Ya-Min Tian,Michael I. Wilson,Janine Gielbert,Simon J. Gaskell,Alex von Kriegsheim,Holger F. Hebestreit,Mridul Mukherji,Christopher J. Schofield,Patrick H. Maxwell,Christopher W. Pugh,Peter J. Ratcliffe +12 more
TL;DR: It is shown that the interaction between human pVHL and a specific domain of the HIF-1α subunit is regulated through hydroxylation of a proline residue by an enzyme the authors have termed Hif-α prolyl-hydroxylase (HIF-PH).
Journal ArticleDOI
C. elegans EGL-9 and mammalian homologs define a family of dioxygenases that regulate HIF by prolyl hydroxylation.
Andrew C. R Epstein,Jonathan M. Gleadle,Luke A. McNeill,Kirsty S. Hewitson,J F O'Rourke,David R. Mole,Mridul Mukherji,Eric Metzen,Michael A Wilson,Anu Dhanda,Ya-Min Tian,Norma Masson,Donald L. Hamilton,Panu Jaakkola,Robert Barstead,Jonathan Hodgkin,Patrick H. Maxwell,Christopher W. Pugh,Christopher J. Schofield,Peter J. Ratcliffe +19 more
TL;DR: Direct modulation of recombinant enzyme activity by graded hypoxia, iron chelation, and cobaltous ions mirrors the characteristics of HIF induction in vivo, fulfilling requirements for these enzymes being oxygen sensors that regulate HIF.
Journal ArticleDOI
Hypoxia inducible factor-alpha binding and ubiquitylation by the von Hippel-Lindau tumor suppressor protein.
Matthew Edward Cockman,Norma Masson,David R. Mole,Panu Jaakkola,Gin-Wen Chang,Steven C. Clifford,Eamonn R. Maher,Christopher W. Pugh,Peter J. Ratcliffe,Patrick H. Maxwell +9 more
TL;DR: It is shown that extracts from VHL-deficient renal carcinoma cells have a defect in HIF-α ubiquitylation activity which is complemented by exogenous pVHL, and this defect was specific for Hif-α among a range of substrates tested.
Journal ArticleDOI
HIF overexpression correlates with biallelic loss of fumarate hydratase in renal cancer: Novel role of fumarate in regulation of HIF stability
Jennifer S. Isaacs,Yun Jin Jung,David R. Mole,Sunmin Lee,Carlos Torres-Cabala,Yuen-Li Chung,Maria J. Merino,Jane B. Trepel,Berton Zbar,Jorge R. Toro,Peter J. Ratcliffe,W. Marston Linehan,Len Neckers +12 more
TL;DR: In this article, a novel fumarate-dependent pathway for regulating HPH activity and HIF protein levels was proposed, which coincides with FH inhibition and upregulation of HIF upregulation.
Journal ArticleDOI
Resolving the fibrotic niche of human liver cirrhosis at single cell level
Prakash Ramachandran,Ross Dobie,John R. Wilson-Kanamori,Elena Dora,Beth E. P. Henderson,N T Luu,N T Luu,Jordan R. Portman,Kylie P. Matchett,M Brice,John A. Marwick,Richard S Taylor,Mirjana Efremova,Roser Vento-Tormo,Neil O. Carragher,Timothy J. Kendall,Jonathan A. Fallowfield,Ewen M Harrison,David R. Mole,David R. Mole,Stephen J. Wigmore,Stephen J. Wigmore,Philip N. Newsome,Philip N. Newsome,Christopher J. Weston,Christopher J. Weston,John P. Iredale,Frank Tacke,Jeffrey W. Pollard,Jeffrey W. Pollard,Chris P. Ponting,John C. Marioni,John C. Marioni,John C. Marioni,Sarah A. Teichmann,Sarah A. Teichmann,Sarah A. Teichmann,Neil C. Henderson +37 more
TL;DR: Analysis of transcriptomes of more than 100,000 single human cells yields molecular definitions for non-parenchymal cell types that are found in healthy and cirrhotic human liver, and identifies markers for scar-associated macrophages and endothelial cells.