Davide Cacchiarelli

TL;DR: Monocle is described, an unsupervised algorithm that increases the temporal resolution of transcriptome dynamics using single-cell RNA-Seq data collected at multiple time points that revealed switch-like changes in expression of key regulatory factors, sequential waves of gene regulation, and expression of regulators that were not known to act in differentiation.

TL;DR: It is demonstrated that linc-MD1 exerts the same control over differentiation timing in human myoblasts, and that its levels are strongly reduced in Duchenne muscle cells, indicating that the ceRNA network plays an important role in muscle differentiation.

TL;DR: A dense network of proteins interacting with METTL3, a component of the methyltransferase complex, is identified, and it is shown that three of them (WTAP, METTL14, and KIAA1429) are required for methylation.

TL;DR: The derivation of GMP-compliant hydrogels from decellularized porcine small intestine which support formation and growth of human gastric, liver, pancreatic and small intestinal organoids are demonstrated.

TL;DR: It is shown that, like LIN28A, LIN28B can function effectively with NANOG, OCT4, and SOX2 in reprogramming to pluripotency and that reactivation of both endogenousLIN28A and LIN28 B loci are required for maximal reprograming efficiency.