Diane E. Kelly

TL;DR: It is shown that previously known and new genes are necessary for optimal growth under six well-studied conditions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment, and less than 7% of genes that exhibit a significant increase in messenger RNA expression are also required for optimal Growth in four of the tested conditions.

TL;DR: It is demonstrated that different molecular mechanisms contribute to a gradual development of fluconazole resistance in C. albicans.

TL;DR: It is shown in two clinical isolates that resistance was caused by defective sterol Δ5,6‐desaturation and growth arrest after fluconazole treatment of C. albicans in clinical conditions is caused by 14α‐methylergosta‐8,24(28)‐dien‐3β,6α‐diol accumulation.

TL;DR: It is shown that cell growth arrest correlates with the accumulation of 14α-methyl-ergosta-8,24(28)-dien-3β,6α-diol in a yeast strain with a sterol 14 α-demethylase gene disruption, which mimics stringent treatment conditions.

TL;DR: This work undertook site-directed mutagenesis of the Candida albicans heterologously expressed in Saccharomyces cerevisiae to probe a model structure for the sterol 14α-demethylase, and made the first example of a single base change in the target enzyme conferring resistance to azoles through reduced azole affinity.