Dirk Aerts

TL;DR: It is shown that wood samples derived from the transgenic trees are more easily processed into ethanol, and CCR down-regulation may become a successful strategy to improve biomass processing if the variability in down- regulation and the yield penalty can be overcome.

TL;DR: Site-saturation libraries of the Pseudomonas fluorescens esterase were created targeting three surface positions to increase its thermostability on the basis of the B-factor iterative test principle andVariants could be identified that showed significantly improved stability without compromising specific activity.

TL;DR: Improving the affinity for alternative acceptors by means of enzyme engineering will be a major challenge for the commercial exploitation of the transglucosylation potential of sucrose phosphorylase.

TL;DR: The stability of the enzyme from Bifidobacterium adolescentis has been significantly improved by a combination of smart and rational mutagenesis, and an increased stability in the presence of organic co-solvents could also be observed, although these effects were most noticeable at low temperatures.

TL;DR: The constitutive expression of sucrose phosphorylases in low‐well plates resulted in a level of activity that is equal or even better than what was achieved by inducible expression, suggesting that the plasmid set with varying constitutive promoters will be an indispensable tool to optimize enzyme expression for high‐throughput screening.