D
Dmitry A. Shagin
Researcher at Russian National Research Medical University
Publications - 31
Citations - 3380
Dmitry A. Shagin is an academic researcher from Russian National Research Medical University. The author has contributed to research in topics: Complementary DNA & cDNA library. The author has an hindex of 17, co-authored 29 publications receiving 3167 citations. Previous affiliations of Dmitry A. Shagin include Russian Academy of Sciences.
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Journal ArticleDOI
Genetic basis for individual variations in pain perception and the development of a chronic pain condition
Luda Diatchenko,Gary D. Slade,Andrea G. Nackley,Konakporn Bhalang,Asgeir Sigurdsson,Inna Belfer,David Goldman,Ke Xu,Svetlana A. Shabalina,Dmitry A. Shagin,Mitchell B. Max,Sergei S. Makarov,William Maixner +12 more
TL;DR: Three genetic variants of the gene encoding catecholamine-O-methyltransferase determine COMT activity in humans that inversely correlates with pain sensitivity and the risk of developing TMD.
Journal ArticleDOI
The mammalian pannexin family is homologous to the invertebrate innexin gap junction proteins.
Ancha Baranova,Dmitry Ivanov,Nadezda Petrash,Anya Pestova,Mikhail Skoblov,Ilya V. Kelmanson,Dmitry A. Shagin,Svetlana Nazarenko,Elena Geraymovych,Oxana Litvin,Anya Tiunova,Timothy L. Born,Natalia Usman,Dmitry B. Staroverov,Sergey Lukyanov,Yury Panchin +15 more
TL;DR: Cloned genes PANX1, PANX2 and PANX3, encoding putative gap junction proteins homologous to invertebrate innexins, which constitute a new family of mammalian proteins called pannexins are revealed, pointing to their important function.
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Simple cDNA normalization using kamchatka crab duplex-specific nuclease
Pavel A. Zhulidov,Ekaterina A. Bogdanova,Alex S. Shcheglov,Vagner Ll,George L. Khaspekov,Valery B. Kozhemyako,Mikhail V. Matz,Ella A. Meleshkevitch,Leonid L. Moroz,Sergey Lukyanov,Dmitry A. Shagin +10 more
TL;DR: A novel simple cDNA normalization method that may be effectively used for samples enriched with full-length cDNA sequences and employed to normalize cDNA from nervous tissues of the marine mollusc Aplysia californica to illustrate further the efficiency of the normalization technique.
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GFP-like Proteins as Ubiquitous Metazoan Superfamily: Evolution of Functional Features and Structural Complexity
Dmitry A. Shagin,Ekaterina V. Barsova,Yurii G. Yanushevich,Arkady F. Fradkov,Konstantin A. Lukyanov,Yulii Aleksandrovich Labas,Tatiana N. Semenova,Juan A. Ugalde,Juan A. Ugalde,Ann Meyers,Jose Manuel Nunez,Edith A. Widder,Sergey Lukyanov,Mikhail V. Matz +13 more
TL;DR: Diverse GFP-like proteins from previously undersampled and completely new sources are described, including hydromedusae and planktonic Copepoda, and a new yellow protein seems to follow exactly the same structural solution to achieving the yellow color of fluorescence as YFP, an engineered yellow-emitting mutant variant of GFP.
Journal ArticleDOI
A Novel Method for SNP Detection Using a New Duplex-Specific Nuclease From Crab Hepatopancreas
Dmitry A. Shagin,Denis V. Rebrikov,Valery B. Kozhemyako,Ilia M. Altshuler,Alex S. Shcheglov,Pavel A. Zhulidov,Ekaterina A. Bogdanova,Dmitry B. Staroverov,Valery A. Rasskazov,Sergey Lukyanov +9 more
TL;DR: A novel assay for single nucleotide polymorphism (SNP) detection based on "duplex-specific nuclease preference" (DSNP), where the DNA region containing the SNP site is amplified and the PCR product mixed with signal probes and DSN differentiates between one-nucleotide variations in DNA.