Don Roach

TL;DR: Gas-liquid chromatography studies of direct esterification of protein amino acids to n-butyl esters have been carried out by as mentioned in this paper for protein amino acid amino acid synthesis.

TL;DR: The development of a gas—liquid chromatographic (GLC) method for the quantitative analysis of amino acids in complex biological substances, specifically blood plasma and urine, has been achieved and data obtained were in excellent agreement with results by classical ion exchange.

TL;DR: Experiments were made to investigate the effect of temperature on the hydrolysis reaction in aqueous 6 N HCl with a view to developing a rapid Hydrolysis procedure and the yields obtained were in good agreement.

TL;DR: Butyl esters separation from protein amino acids, using acid washed Chromosorb W of various mesh sizes was performed in this paper, where acid washed chromosorb was used to remove the butyl ester from proteins.

TL;DR: Quantitative gas-liquid chromatography of histidine, using N-TFA n-butyl ester derivatives and histidine converted trimethylsilyl derivative is described in this paper.

TL;DR: The amino acid analysis technology needs further simplification and automation of the hydrolysis, chromatography and detection steps to withstand the pressure exerted by the other technologies.

TL;DR: Using phenylisothiocyanate as the reagent, detection limits under 1 pmol can be routinely achieved, allowing the analysis of submicrogram protein samples, giving the analyst the first realistic alternative to ion-exchange analysis without compromising desirable features of the traditional methodology.

TL;DR: The use of ultrasonication to remove dissolved air while pulling a vaccumm on the sample solution prior to hydrolysis assured a good recovery for methionine and cystine as discussed by the authors.

TL;DR: A gas-liquid chromatographic procedure is described which will separate and quantitate the seventeen amino acids typically found in protein acid hydrolyzates and displays a characteristic and unique [M- 57] fragment ion for each amino acid which often dominates the mass spectrum.

TL;DR: Based on solvent-free gas chromatography of the n -propyl, procedures are described for the routine determination of amino acid profiles in biological samples including protein hydrolysates, plant tissue extracts, urines and sera, indicating that a coefficient of variation of 2–5% is attainable.