Donald J. Hanahan

TL;DR: The effects of the ionic strength and pH of the hemolyzing solution on the hemoglobin content of human erythrocyte ghosts were studied in phosphate buffers and suggest an electrophysical interaction of hemoglobin with membrane constituents.

TL;DR: Human red cell lipids have been studied in a series of normal individuals and evidence is presented that the total amount of lipid phosphorus per average red cell is 1.40 X lo-" mg and that most extraction procedures fail to extract 870 or more of red cell phospholipid.

TL;DR: The procedure developed is simple, rapid, and generally applicable t o lipids, and the results did not affect the validity of the method.

TL;DR: It is found that as the surface charge of the lipid lamellae is increased, the amount of cation per μmle of lipid increases, and the phospholipid liquid crystalline structures appear to “bind” or “capture” cations.

TL;DR: The effects of the ionic strength and pH of the hemolyzing solution on the hemoglobin content of human erythrocyte ghosts were studied in phosphate buffers and suggest an electrophysical interaction of hemoglobin with membrane constituents.

TL;DR: The partition of proteins during phase separation in solutions of Triton X-114 is investigated and integral membrane proteins with an amphiphilic nature are recovered in the detergent phase.