Dorota Garczarczyk

TL;DR: It is concluded that PKCα might be an important target for the antiangiogenic effects of tubulin antagonism, and the data from the kinase array suggest that different tubulin antagonists might have individual intracellular actions.

TL;DR: Investigation of the effects of constitutively active rat PKCepsilon in HeLa cells in a doxycycline-inducible vector demonstrates that PKCEPsilon can induce distinctly different signaling from the Golgi and from the plasma membrane.

TL;DR: A pharmacophore model from the peptide EAVSLKPT is derived, a fragment of PKCε that inhibits the interaction ofPKCε and receptor for activated C-kinase 2 (RACK2) in vitro, leading to the discovery of a series of thienoquinolines that disrupt the interaction with RACK2 in vitro.

TL;DR: The small organic molecule BAS 02104951 can be used as selective inhibitor of PKCε in cells not expressing PKCη and may serve as a basis for the rational development of a selective inhibitors ofPKCε or PKC ε, or for an inhibitor of the PKCα/RACK2 interaction.

TL;DR: The presence of unphosphorylated PKCgamma in HT29 cells, and its complete absence in Caco2 cells demonstrates a cell type-dependent differential coupling of Thr(514)- phosphorylation with de novo synthesis of PKCGamma in colon cancer cells.