Showing papers by "Douglas B. Kell published in 1984"
TL;DR: A fifth postulate is proposed, namely that of the coupling unit, to the four existing postulates of 'delocalized protonic coupling' and it is shown that, with this postulate, protono-coupling can again account for most experimental observations.
192 citations
TL;DR: This poster presents a probabilistic study of the phytochemical properties of mitochondria, the building block of DNA that acts as a “spatially aggregating force” to form DNA.
81 citations
TL;DR: The random two-dimensional diffusion coefficient of many membrane proteins estimated from a variety of biophysical measurements is much greater than must be inferred from certain more biochemically based experiments in prokaryotes.
40 citations
TL;DR: It is concluded that the observable protonmotive activity of the respiratory chain of P. denitrificans in the absence of SCN- is directly influenced by the state of the H+-ATP synthetase in the cytoplasmic membrane of this organism.
38 citations
TL;DR: It is believed that the archipelago effect, in which the proteins are treated as hard disks, although not entirely to be dismissed from consideration, would not be resolved.
7 citations
2 citations
TL;DR: The bactericidal activity of Tinopal AN was shown to be due to a mechanism entirely independent of its inhibitory effects upon NADH dehydrogenase which were reported previously, and RNA and protein synthesis were immediately and markedly inhibited.
Abstract: The bactericidal activity of Tinopal AN [1,1-bis(3,N-5-dimethyl-benzoxazol-2-yl)-methine p-toluene sulphonate] was shown to be due to a mechanism entirely independent of its inhibitory effects upon NADH dehydrogenase which were reported previously. Whereas the compound had no significant effect upon DNA synthesis in Escherichia coli D22, RNA and protein synthesis were immediately and markedly inhibited. In confirmation, Tinopal AN caused an immediate cessation in inducible β-galactosidase synthesis in the same organism. An in vitro assay of the transcription of calf-thymus DNA by purified E. coli RNA polymerase showed that this process was inhibited by Tinopal AN.
2 citations
01 Jan 1984
TL;DR: The chemiosmotic hypothesis for oxidative and photosynthetic phosphorylation has given a powerful impulse to the study and the understanding of membrane associated electron transfer and its coupling to ATP formation.
Abstract: There is no doubt that the chemiosmotic hypothesis for oxidative and photosynthetic phosphorylation (Mitchell, 1966)has given a powerful impulse to the study and the understanding of membrane associated electron transfer and its coupling to ATP formation. The hypothesis has been of particular importance in emphasizing the vectorial aspects of oxidoreduction reactions in energy conserving membranes and the relevance of protonic activity and electrostatic potential differences for the coupling of energy transducing enzymes. Many basic features of the hypothesis have been experimentally substantiated; such features include the asymmetric distribution of redox carriers across the membranes, the formation of transmembrane differences in pH and/or in electrostatic potential by the various redox complexes and by the ATPase, the possibility of driving ATP synthesis by artificially imposed ΔpH or diffusion potentials of ions, or by a combination of these two forces, and the possibility of coupling chemiosmotically in reconstituted vesicles heterologous energy transducing complexes (Boyer et al., 1977; Ferguson, Sorgato, 1982).