Showing papers by "Douglas B. Kell published in 1989"
TL;DR: Article de synthese sur le role antimicrobien du dioxyde de carbone au niveau des aliments, and sur the croissance microbienne au cours de divers procedes de fermentation.
Abstract: Article de synthese sur le role antimicrobien du dioxyde de carbone au niveau des aliments (eau, lait, poisson, fruits, legumes), et sur la croissance microbienne au cours de divers procedes de fermentation
482 citations
01 Jan 1989
TL;DR: The present method provides a simple and rapid means for assessing the biocompatibility of solvents used in biotransformations.
Abstract: Dielectric spectroscopy provides a convenient means of determining the degree of intactness of biological cells. 4-terminal dielectric measurements of suspensions of Saccharomyces cerevisiae at 0.4 MHz show that, as with all other biological cells, these organisms possess a substantial β-dispersion. The additional of octanol to such suspensions causes a rapid decrease in the electrical capacitance of the suspension, which parallels the cellular viability as determined by methylene blue staining. The kinetics of cell death are determined in part by the rate of dissolution of the organic solvent in the aqueous phase. The toxicity of several organic solvents to S. cerevisiae is studied using this technique, and is found to be dependent upon the polarity of the solvent. The present method provides a simple and rapid means for assessing the biocompatibility of solvents used in biotransformations.
55 citations
TL;DR: In this paper, the authors discuss the trade-off between resolution, precision, accuracy, response time, ease of use and expense of mass spectrometers for potentiometric devices.
53 citations
TL;DR: This method was used for a detailed study of the enyzmology of the overall reaction catalysed by pyruvate synthase in Clostridium sporogenes and could be utilised to harness such reductive carboxylations for commercial biotransformations of xenobiotics.
4 citations
TL;DR: The covalently immobilised lipase maintained 90–100% of its initial activity over a period of 150 days and could convert R,S-citronellol and butyric acid to citronellyl butyrate at rates in the range 7–51 μmol.
Abstract: Lipase fromC cylindracea was covalently immobilised to a number of surface-treated ceramic supports (3–10 mg (g dry wt support)−1) At room temperature, the immobilised lipase could convert R,S-citronellol and butyric acid to citronellyl butyrate at rates in the range 7–51 μmol (mg lipasemin)−1 The lipase maintained 90–100% of its initial activity over a period of 150 days
3 citations