D
Douglas J. Demarini
Researcher at University of North Carolina at Chapel Hill
Publications - 3
Citations - 5838
Douglas J. Demarini is an academic researcher from University of North Carolina at Chapel Hill. The author has contributed to research in topics: Protein subunit & Gene. The author has an hindex of 3, co-authored 3 publications receiving 5499 citations.
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Journal ArticleDOI
Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
Mark S. Longtine,Amos Mckenzie,Douglas J. Demarini,Nirav Shah,Achim Wach,Arndt Brachat,Peter Philippsen,John R. Pringle +7 more
TL;DR: A new set of plasmids that serve as templates for the PCR synthesis of fragments that allow a variety of gene modifications that should further facilitate the rapid analysis of gene function in S. cerevisiae.
Journal ArticleDOI
The septins: Roles in cytokinesis and other processes
Mark S. Longtine,Douglas J. Demarini,Maria L. Valencik,Omayma S Al-Awar,Hanna Fares,Claudio De Virgilio,John R. Pringle +6 more
TL;DR: The septins are a novel family of proteins that were first recognized in yeast as proteins associated with the neck filaments and appear to be essential for this process in both fungal and animal cells.
Journal ArticleDOI
Inactivation of the yeast Sen1 protein affects the localization of nucleolar proteins
TL;DR: It is verified that a region in SEN1 containing a putative nuclear localization signal sequence (NLS) is necessary for nuclear targeting and found that inactivation of Sen1p by temperature shift of a strain carrying sen1-1 leads to mislocalization of two nucleolar proteins, Nopt and Ssb1.