D
Duarte M. F. Prazeres
Researcher at University of Lisbon
Publications - 72
Citations - 3738
Duarte M. F. Prazeres is an academic researcher from University of Lisbon. The author has contributed to research in topics: Plasmid & Plasmid preparation. The author has an hindex of 32, co-authored 69 publications receiving 3571 citations. Previous affiliations of Duarte M. F. Prazeres include Instituto Superior Técnico & Technical University of Lisbon.
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Book ChapterDOI
Horseradish peroxidase: a valuable tool in biotechnology.
Ana Azevedo,Verónica C. Martins,Duarte M. F. Prazeres,Vojislav Vojinović,Joaquim M. S. Cabral,Luís P. Fonseca +5 more
TL;DR: This review addresses the applications of peroxidases, especially HRP, in the environmental and health care sectors, and in the pharmaceutical, chemical and biotechnological industries.
Journal ArticleDOI
Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: problems and bottlenecks
Duarte M. F. Prazeres,Guilherme N. M. Ferreira,Gabriel A. Monteiro,Charles L. Cooney,Joaquim M. S. Cabral +4 more
TL;DR: There are several problems and bottlenecks associated with the design and operation of large-scale processes for the production of pharmaceutical-grade plasmid DNA for gene therapy.
Journal ArticleDOI
Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications.
TL;DR: A review of downstream operations for large-scale purification of plasmid DNA, describing their principles and the strategy used to attain a final product that meets specifications is presented in this article.
Journal ArticleDOI
Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography.
Maria Margarida Diogo,João A. Queiroz,Gabriel A. Monteiro,Gabriel A. Monteiro,S. A. M. Martins,S. A. M. Martins,Guilherme N. M. Ferreira,Duarte M. F. Prazeres +7 more
TL;DR: A new method is described for the purification of a cystic fibrosis plasmid vector of clinical grade, which includes an ammonium sulfate precipitation followed by hydrophobic interaction chromatography (HIC) using a Sepharose gel derivatized with 1,4‐butanediol‐diglycidylether.
Journal ArticleDOI
Preparative purification of supercoiled plasmid DNA using anion-exchange chromatography.
TL;DR: The use of Q-Sepharose columns (1, 10 and 40 ml) for the preparative purification of plasmid pUC18 enabled the isolation of supercoiled plasmids from low-M(r) RNA, cDNA and plasmID variants and anion-exchange HPLC on a Poros QE 20 column was used to quantifyplasmid yield.