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E. W. Yemm

Bio: E. W. Yemm is an academic researcher. The author has contributed to research in topics: Ninhydrin. The author has an hindex of 1, co-authored 1 publications receiving 1815 citations.
Topics: Ninhydrin

Papers
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Journal ArticleDOI
01 Jan 1955-Analyst
TL;DR: A systematic review of the ninhydrin colour test for amino-acids is given in this article, with a note on its use in the identification of protein hydrolysates.
Abstract: A systematic review of the mechanism and application of the ninhydrin colour test for amino-acids, with a note on its use in the identification of protein hydrolysates. -- AATA

1,967 citations


Cited by
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Journal ArticleDOI
TL;DR: The present method avoids the necessity for the preparation of solutions of reduced ninhydrin, which is an unstable reagent difficult to prepare and impracticable to store.

2,124 citations

Book ChapterDOI
TL;DR: This chapter discusses the chemical analysis of microbial cells and wet- and dry-weight determinations of bacterial samples and assay of total cell numbers are described, because analytical results must refer to one or other of these values.
Abstract: Publisher Summary This chapter discusses the chemical analysis of microbial cells. The preparation of material for analysis is discussed, because changes in the chemical composition of cells may occur as a result of the washing and storage conditions used. Wet- and dry-weight determinations of bacterial samples and assay of total cell numbers are described, because analytical results must refer to one or other of these values. Selection of an analytical procedure is a subjective process, because the number of suitable methods is large and each will have different merits and defects. Primary considerations are sensitivity, specificity, reproducibility, and absolute accuracy. Automatic methods for performing biochemical analyses, already widely accepted in hospitals and in industry, are beginning to make their way into the research laboratory. All automatic analyzers developed so far may be classified as either “continuous-flow” or “discrete” types. All of them use colorimetric methods exclusively and contain some form of automatic colorimeter for final read-out. The first and best-known is the Technicon “AutoAnalyzer,” which is of the continuous-flow type.

1,193 citations

Journal ArticleDOI
TL;DR: In this paper, the rates of change of film pressure (π) and surface concentration (Γ) of protein during adsorption of β-casein, bovine serum albumin (BSA), and lysozyme at the air-water interface have been monitored by the Wilhelmy plate and surface radioactivity methods, respectively.

773 citations

Journal ArticleDOI
TL;DR: In this paper, an improved colorimetric determination of amino acids with the use of ninhydrin is described, and the method is shown to improve the color of amino acid sequences.

742 citations

Journal ArticleDOI
TL;DR: The present report describes the isolation, partial purification, and characterization of phenylalanine deaminase, an enzyme from barley that converts Lphenylalanines to cinnamic acid and ammonia and finds that monocotyledons but not dicotyles can readily convert n-tyrosine to lignin.

629 citations