Eric Spooner

TL;DR: It is proposed that the relative strengths of the rheb- and PRAS40-mediated inputs to m TORC1 set overall pathway activity and that insulin activates mTORC1 through the coordinated regulation of both.

TL;DR: The finding that this family of demethylases generates different methylated states at the same lysine residue provides a mechanism for fine-tuning histone methylation.

TL;DR: sortagging (sortase-mediated transpeptidation) is a versatile chemoenzymatic system for site-specific labeling of proteins with small (<2 kDa) probes and is applied to proteins in vitro and on the surface of living cells.

TL;DR: Using insertional mutagenesis to develop a screening method to generate null alleles in a human cell line haploid for all chromosomes except chromosome 8, host factors essential for infection with influenza and genes encoding important elements of the biosynthetic pathway of diphthamide, which are required for the cytotoxic effects ofdiphtheria toxin and exotoxin A are identified.

TL;DR: Although cathepsin L generated the requisite TLR9 cleavage products in a cell-free in vitro system, several proteases influenced TLR7 cleavage in intact cells, and Lysosomal proteolysis contributes to innate immunity by facilitating specific cleavage ofTLR9.