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Author

Ermin Rachmawati

Other affiliations: Lampung University
Bio: Ermin Rachmawati is an academic researcher from University of Brawijaya. The author has contributed to research in topics: Foam cell & Chemistry. The author has an hindex of 3, co-authored 7 publications receiving 15 citations. Previous affiliations of Ermin Rachmawati include Lampung University.

Papers
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Journal ArticleDOI
TL;DR: The role of miR-155–5p in foam cell atherosclerosis inhibition is strongly suggested and Eleven original studies were included in the review and strongly suggest the role of MiRNAs targeting three genes involved in foamcell atheros sclerosis inhibition.

9 citations

Journal ArticleDOI
25 Apr 2012
TL;DR: Kanker serviks merupakan penyebab utama kedua kematian kanker yang dimediasi oleh stabilisasi p53 pada pertumbuhan kanker serviks, Sebuah percobaan in vitro dilakukan dengan menggunakan kultur sel HeLa.
Abstract: Kanker serviks merupakan penyebab utama kedua kematian kanker pada wanita di seluruh dunia, menyebabkan 240.000 kematian setiap tahunnya. Ekstrak daun Annona muricata berpotensi baik sebagai obat anti kanker . T ujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstrak etanol daun sirsak (Annona muricata) dalam menghambat dan menginduksi aktivasi apoptosis yang dimediasi oleh stabilisasi p53 pada pertumbuhan kanker serviks. Sebuah percobaan in vitro dilakukan dengan menggunakan kultur sel HeLa. Penghambatan proliferasi diukur dengan MTT assay. Apoptosis dideteksi dengan menggunakan flowcytometry Annexin V Biotin Kit Apoptosis Detection. Konfirmasi dan perhitungan ekspresi p53 ditentukan oleh imunocytochemistry. Ekstrak etanol dari daun Annona muricata secara efektif menghambat proliferasi sel HeLa dengan dosis tertentu pada paparan 48 jam. Nilai IC50 ekstrak etanol daun Annona muricata adalah 111,75 ug/ml. 200 mg/ml ekstrak menginduksi apoptosis sel HeLa tergantung dari lama pemaparan. Ekstrak ini juga meningkatkan ekspresi p53 sel HeLa tergantung dosis pemberian (25, 50, 100, 200 μg/ml) dan lama pemberian (24 dan 48 jam). Dapat disimpulkan bahwa ekstrak etanol daun sirsak menghambat pertumbuhan sel HeLa dan menginduksi apoptosis. Mekanisme ekstrak daun sirsak menghambat proliferasi dan apoptosis inducing berhubungan dengan stabilisasi dan aktivasi p53.Kata Kunci: Daun Annona muricata, apoptosis, ekstrak etanol, proliferasi, ekspresi p53

8 citations

27 Mar 2018
TL;DR: Kunci et al. as discussed by the authors dilaksanakan di salah satu SDN di Natar bulan Oktober-November 2015, dilakukan dengan menggunakan kuesioner, penilaian status gizi melalui pengukuran tinggi badan and berat badan serta uji laboratorium dengan metode apung untuk penilaIAN infeksi kecacingan.
Abstract: Prevalensi kecacingan di Indonesia relatif tinggi yaitu sebesar 22,6% dengan mayoritas penderita adalah balita dan murid sekolah dasar. Banyak faktor yang dapat menyebabkan kecacingan adalah personal hygiene yang buruk. Kecacingan dapat menyebabkan status gizi anak menjadi kurang. Penelitian ini bertujuan untuk mengetahui hubungan antara personal hygiene dan status gizi dengan infeksi kecacingan pada siswa sekolah dasar negeri di Natar. Jenis penelitian ini adalah analitik observasional dengan menggunakan rancangan cross sectional. Penelitian ini dilaksanakan di salah satu SDN di Natar bulan Oktober-November 2015. Subjek penelitian adalah 50 orang siswa kelas 1 sampai kelas 6. Hubungan antara personal hygiene dan status gizi dengan infeksi kecacingan diuji dengan uji statistik Chi-square dengan menggunakan software komputer. Penilaian personal hygiene dilakukan dengan menggunakan kuesioner, penilaian status gizi melalui pengukuran tinggi badan dan berat badan serta uji laboratorium dengan metode apung untuk penilaian infeksi kecacingan. Dari 50 responden, 62% mengalami infeksi kecacingan dengan jenis Ascaris lumbricoides. Status gizi berdasarkan IMT/U kategori kurus 10%, normal 80%, gemuk 6% dan sangat gemuk 4%. Prevalensi kebersihan kuku 64%, alas kaki 60%, dan cuci tangan 62% kategori tidak baik. Terdapat hubungan antara kebersihan kuku, penggunaan alas kaki, dan kebiasaan cuci tangan dengan infeksi kecacingan dengan masing-masing p value 0,002, 0,009, dan 0,000. Tidak terdapat hubungan antara status gizi dengan infeksi kecacingan dengan p value 0,80. Terdapat hubungan antara personal hygiene dengan infeksi kecacingan namun tidak terdapat hubungan antara status gizi dengan infeksi kecacingan. Kata Kunci: infeksi kecacingan, personal hygiene, status gizi

3 citations

Proceedings ArticleDOI
25 May 2021
TL;DR: It is concluded that holy basil leaves extract may exert anti-atherosclerosis inhibition through attenuation of fatty streak formation through absorption in aorta wall.
Abstract: Atherosclerosis is the etiology of coronary heart disease (CHD) that contributes to the highest mortality rate of non-communicable disease groups in Indonesia. The early stage of this process is marked by the presence of fatty streaks lesion in the artery wall. This study aimed to investigate the effect of holy basil leaves (Ocimum sanctum L.) to suppress the formation of the fatty streak as previous study prove the reduced lipid levels, ROS, and inflammation after treated with this extract. 25 adult male New Zealand rabbits age 4-month weight 2500-3000 g fed with a high-fat diet (HFD) and 10 mg/kgBW/day Holy Basil Extract (HBE), 25 mg/kgBW/day HBE, 50 mg/kgBW/day HBE. The negative control group was received only HFD. For the experimental standard, one group provided with standard diet rabbits were included. The fatty streak was measured in aorta wall after 45 days of treatment by immunohistochemistry method and quantified using Image J software. The results showed that there was no significant difference in the fatty streak area between each group (p > 0.05). Still, there was a trend of decreasing fatty streak area dose dependent manner (negative control 5802.21+3690.41 µm2, P1 5154.69 + 3990.79 µm2, P2 4938.31 + 3690.18 µm2, and P3 3611.68 + 4092.96 µm2) even though it was not statistically significant (p > 0.05). This paper conclude that holy basil leaves extract may exert anti-atherosclerosis inhibition through attenuation of fatty streak formation.

2 citations

Journal ArticleDOI
TL;DR: In this paper, decaffeinated coffee and green tea extract (DCGTE) has been suggested to have a role in foam cell inhibition, through modulation of the inflammation process and cholesterol metabolism in macrophage colony stimulating factor (M-CSF) and oxidized low-density lipoprotein (oxLDL)-exposed macrophages.
Abstract: Background: Foam cells are markers of atherosclerosis and characterise advanced atherosclerotic plaque, stimulated by inflammation caused by high lipid levels in macrophages. The combination of decaffeinated coffee and green tea extract (DCGTE) has been suggested to have a role in foam cell inhibition. Objective : we investigated the inhibiting role of DCGTE against foam cell formation, through modulation of the inflammation process and cholesterol metabolism in macrophage colony stimulating factor- (M-CSF) and oxidized low-density lipoprotein (oxLDL)-exposed macrophages. Methods : Coffee and green tea were extracted by filtration and infusion respectively, and underwent decaffeination using active carbon and blanching methods, respectively. Cells were administered 160/160 and 320/320μg/ml of DCGTE. Foam cell formation was observed using a light microscope after staining with Oil Red O (ORO), and the accumulation of lipids in macrophages with ELISA. Observations of lipid influx and efflux were determined through semiquantitative cluster differentiation 36 (CD36) and ATP binding cassette transporter A1 (ABCA1) expression through immunofluorescence. The inflammation process was quantified using inflammatory/anti-inflammatory markers, e.g., tumor necrosis factor α (TNFα) and interleukin 10 (IL10) with ELISA. Peroxisome proliferator activated response γ (PPARγ) expression and activity were assessed with PCR and ELISA, respectively. The expression of microRNA 155 (miR-155) was examined using qPCR. Results: DCGTE at the above concentrations tended to reduce foam cell numbers, significantly inhibited lipid accumulation (p=0.000), reduced CD36 expression (p=0.000) and TNFα secretion (p=0.000) in Raw264.7 exposed to M-CSF 50ng/ml and oxLDL 50μg/ml. PPARγ expression (p=0.00) and activity (p=0.001), miR-155 relative expression (p=0.000), and IL10 production (p=0.000) also increased. Conclusion: DCGTE lowered foam cell numbers, possibly through attenuation of the inflammatory process and improvement of lipid/efflux mechanisms in M-CSF and oxLDL-stimulated Raw264.7 cells, via upregulation of PPARγ and miR-155. Our results suggest DCGTE may help prevent atherosclerosis-based diseases.

1 citations


Cited by
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Journal ArticleDOI
TL;DR: Results suggest that signaling from PI3K/pAKT can lead to radiation resistance in LACC, and a significant association was found between the level of pAKT expression and local recurrence.
Abstract: Objective:The aim of this study was to investigate the association of phosphorylated AKT (pAKT) expression and radiation resistance in cervical cancer. Methods:A retrospective review was made of the records of 25 women who received primary radiation therapy due to locally advanced cervical cancer (LACC) with FIGO stage IIB-IVA. Nine patients regarded as radiation resistant developed local recurrences with a median progression free interval of 10 months. Sixteen patients did not show local recurrences, and were regarded as a radiation sensitive group. Using pretreatment paraffin-embedded tissues, we evaluated pAKT expression by immunohistochemistry. Results:A significant association was found between the level of pAKT expression and local recurrence. Immunohistochemical staining for pAKT was significantly more frequent in the radiation resistant than in the radiation sensitive group (p=0.007). The mean progression free survival (PFS) was 84 months for patients with pAKT negative staining (17 cases) and 44 months for patients with pAKT positive expression (8 cases)(p=0.015). Conclusion:These results suggest that signaling from PI3K/pAKT can lead to radiation resistance in LACC.

29 citations

Journal Article
TL;DR: Findings reveal a new regulatory pathway of YY1/HDACs/miR-155/HBP1 in macrophage-derived foam cell formation during early atherogenesis and suggest that miR- 155 is a potential therapeutic target for atherosclerosis.
Abstract: Aims: MicroRNAs (miRNAs) play key roles in inflammatory responses of macrophages. However, the function of miRNAs in macrophage-derived foam cell formation is unclear. Here, we investigated the rol...

18 citations

Journal ArticleDOI
TL;DR: Overall, this study suggests that SLE patients have higher plasma/serum PRL level, but with a regional difference.
Abstract: Background: Prolactin (PRL), a polypeptide hormone produced by the pituitary gland, is involved in the regulation of humoral and cell mediated immune responses. PRL levels have been investigated in several autoimmune diseases including systemic lupus erythematosus (SLE), however, yielded different and inconsistent results. This study aims to derive a more precise evaluation on plasma/serum PRL levels in SLE patients, as well as the potential influential factors.Methods: Studies published from 1 January 1987 to 31 December 2015 in English, which comparing plasma/serum PRL levels between SLE group and control group were searched in PubMed, EMBASE and The Cochrane Library databases. Pooled standard mean difference (SMD) with 95% confidence interval (CI) was calculated by fixed-effects or random-effect model analysis. Heterogeneity test was performed by the Q statistic and quantified using I2, publication bias was evaluated using a funnel plot and Egger’s linear regression test.Results: Five-hundred a...

8 citations