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Etty Widayanti

Bio: Etty Widayanti is an academic researcher from YARSI University. The author has contributed to research in topics: Cancer cell & Telomerase. The author has an hindex of 2, co-authored 8 publications receiving 13 citations.

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DOI
19 Aug 2014
TL;DR: Ethanolic extract of Typhonium flagelliforme Lodd has cytotoxic effects against HeLa cells and MCF-7 cells, and the cytot toxic effects against MCF -7 cells are greater than the cytosome inactivating proteins capable of cutting the DNA or RNA of cancer cells and blocking the growth ofcancer cells.
Abstract: BACKGROUND Cancer is one of the causes of high mortality. Breast and cervical cancers are two of the most frequent cancers affecting women around the world, including Indonesia. Natural materials such as rodent tuber (Typhonium flagelliforme) have anticancer potentials. The rodent tuber extract contains ribosome inactivating proteins (RIPs) capable of cutting the DNA or RNA of cancer cells and blocking the growth of cancer cells. The purpose of this study was to evaluate the cytotoxic effects of Typhonium flagelliforme Lodd extract on HeLa cervical cancer and Michigan Cancer Foundation-7 (MCF-7) breast cancer cells. METHODS Subjects were cultured cell lines of HeLa cells in Rosswell Park Memorial Institute (RPMI) and of MCF-7 cells in Dulbecco’s Minimum Essential Medium (DMEM). Rodent tuber ethanolic extract was diluted in dimethyl sulfoxide (DMSO). The cytotoxicity assay used the 3-(4,5-dimethyl thiazol-2-yl,5-diphenyl) tetrazolium bromide (MTT) method. Absorbance was read in an ELISA reader at a wavelength of 595 nm. RESULTS Rat tuber extract at all dilutions (500; 250; 125; 62.5; 31.25; 15.625;7.81; 3.9 i g/ mL) showed cytotoxic effects against HeLa and MCF-7 cells. Higher concentrations of the extract gave a higher proliferation inhibition effect. Calculated IC50 values of the extract by probit analysis were 30.19 ig/mL against HeLa cells and 5.586 i g/mL against MCF-7 cells. CONCLUSIONS Ethanolic extract of Typhonium flagelliforme Lodd has cytotoxic effects against HeLa cells and MCF-7 cells. The cytotoxic effects against MCF-7 cells are greater than the cytotoxic effects against HeLa cells.

10 citations

Journal ArticleDOI
TL;DR: It was concluded that rodent tuber Extract ( Typhonium flagelliforme Lodd) can reduce the expression of telomerase in Raji cells, so that the rodent tuber extract has potential as an anticancer through theexpression of telomersase.
Abstract: Cancer cells have a relatively high telomerase activity compared to normal cells, so the cancer cells have the ability to continue to proliferate and undergo mitosis uncontrolled. Telomerase is an enzyme which responsible for telomere length, a DNA segment that is at the end of eukaryotic cell chromosomes. Telomeres and telomerase play a role in the incidence of carcinogenesis. Natural materials such as taro mice ( Typhonium flagelliforme ) have potential as anticancer. The purpose of the study was to determine the effects of plant extracts of rat taro on the expression of telomerase in cancer cell Raji. The research method is experimental studies in some form cancerous cell culture cell line, Raji. Used as a control normal cells is Vero cell. The Culture medium used RPMI for Raji cell and M199 for Vero cell. The study consisted of three groups, control, doses of 1 ½ IC50 and IC50 doses. Expression of telomerase enzyme was measured by the Immunohystochemistry method (IHC). The results showed that the expression of telomerase in cancer cells showed values significantly higher than the normal cells (Vero). Giving mice taro plant extracts ( Typhonium flagelliforme ) were able to decrease the expression of telomerase significantly in both treatment doses. It was concluded that rodent tuber extract ( Typhonium flagelliforme Lodd) can reduce the expression of telomerase in Raji cells, so that the rodent tuber extract ( Typhonium flagelliforme Lodd) has potential as an anticancer through the expression of telomerase. Keywords : telomerase, IHC, Typhonium flagelliforme

4 citations

Journal ArticleDOI
03 May 2016
TL;DR: It is shown that Typhonium flagelliforme may have anti-cancer activity, necessitating further investigations, and is capable of decreasing telomerase expression more effectively in cervical cancer cells than in breast cancer cells.
Abstract: Background Cancer cells have a relatively high telomerase activity compared to normal cells, so that cancer cells have the ability for continued proliferation and uncontrolled mitosis. Telomerase is an enzyme responsible for the length of telomeres, DNA segments located at the ends of eukaryotic chromosomes. Natural materials such as rodent tuber ( Typhonium flagelliforme ) have anticancer potential. The purpose of the present study was to determine the effects of Typhonium flagelliforme extract on telomerase expression in HeLa cervical cancer and T47D breast cancer cells. Methods This experimental laboratory study was conducted on cultured HeLa and T47D cancer cell lines, with normal Vero cells as controls, and using RPMI and M199 culture media. The study comprised three groups, i.e. controls, and groups receiving Typhonium flagelliforme extract at doses of ½ IC50 and IC50. Telomerase expression was measured by immunohistochemistry (IHC). Analysis of variance and LSD multiple comparison test were used to analyze the data. Results Telomerase expression in cancer cells showed significantly higher values compared to normal Vero cells. Typhonium flagelliforme extract was capable of significantly decreasing telomerase expression in cancer cells receiving the extract. Conclusion Typhonium flagelliforme extract at different doses is capable of decreasing telomerase expression more effectively in cervical cancer cells than in breast cancer cells. This study shows that Typhonium flagelliforme may have anti-cancer activity, necessitating further investigations.

2 citations

Journal ArticleDOI
TL;DR: It was concluded that physical exercise in athletes KONI at the age of 15- 20 years had no effect on telomere length in leukocytes, and information about the telomeres length in Indonesian athletes at an early age is provided.
Abstract: Telomeres are strands of non coding DNA at the ends of chromosomes that have the primary function to protect DNA from damage and maintain chromosomal stability. Physical exercise will increase the antioxidant activity can increase telomere proteins, lengthen telomeres and or protein networks associated with telomere so that the telomere remains long, or stopping telomere shortening. Telomere length was also associated with age. The purpose of the research was to determine telomere length of leukocyte cells in the KONI (Indonesian National Sports Committee) athletes in Jakarta. The research method is descriptive, by measuring telomere length using quantitative PCR on leukocyte cells. Samples are KONI athletes from several sports, including men and women athletes, with ages between 15-20 years. Used a control group (not athletes) is students of the Faculty of Medicine, University of YARSI. The results showed that there was no significant difference (p> 0.05) between telomere length group of athletes with the control group in both sexes. Similarly, telomere length between athlete male with female athletes also showed no significant difference (p> 0.05). It was concluded that physical exercise in athletes KONI at the age of 15- 20 years had no effect on telomere length in leukocytes. The results of this study provide information about the telomere length in Indonesian athletes at an early age.

2 citations

Journal ArticleDOI
TL;DR: It was concluded, that the cancer cells have telomerase expression of specific and different from each other, depending on the type of cell.
Abstract: Background: Cancer is a disease that gets serious attention in the medical world. This is due to the ever increasing number of patients and there has been no effective way to treat. Cancer cells have telomerase activity is relatively high compared to normal cells, so the cancer cells have the ability to continue to proliferate. Cancer cells undergo uncontrolled mitosis and have high telomerase activity compared to cells normal. Telomerase is an enzyme responsible for telomere length, a segment of DNA that is the tip of chromosomes in eukaryotic cells. Telomeres are associated with the process of ageing and carcinogenesis. The purpose of this study was to determine the expression of telomerase in some cells such as breast cancer, cervical cancer, and lung cancer. Methods: The research method is experimental studies in several cancer cell cultures in the form of cell line. Cancer cells used were: HeLa (cervical cancer), MCF7 and T47D (breast cancer), WiDr (lung cancer), and Raji (lymphoma) with culture medium RPMI, DMEM, and M199. Vero cells is used (fibroblast cells) as a control (normal cells). Expression of telomerase enzyme was measured by the Immunohystochemistry (IHC) method. Results: The results showed that the cancer cells have activity/higher telomerase expression were highly significant ( p <0.01) compared to normal cells (Vero cells). Similarly, the expression of telomerase in HeLa versus WiDr, WiDr versus T47D, T47D versus Raji, and Raji versus MCF7 also showed highly significant differences ( p <0.01). Telomerase expression between cancer cells that showed significant difference (HeLa cells versus Raji cells; HeLa cells versus MCF7 cell; T47D cells versus MCF7 cells) ( p <0.05). No significant difference was found in the group of HeLa cells versus T47D, WiDr versus Raji cells, and WiDr versus MCF7. Conclusions: It was concluded, that the cancer cells have telomerase expression of specific and different from each other, depending on the type of cell. T47D breast cancer cells have telomerase expression of the highest, followed by cervical cancer cells (HeLa). Lung cancer cells (WiDr) with cell lymphoma (Raji) has almost the same expression and both have lower expression.

2 citations


Cited by
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Journal Article
TL;DR: In vitro studies on medicinal plants available in Asian countries, including Aloe vera, Alpinia galanga, Centella asiatica, Andrographis paniculata, Curcuma longa, Morinda citrifolia, Trigonella foenum-graecum, Zingiber officinale, Pereskia bleo and Typhonium flagelliforme are summarized.
Abstract: Breast cancer is the most common cause of cancer in women, and the side effects reported for conventional treatments have motivated scientists to investigate the anticancer properties of natural products such as medicinal plants. This review summarizes the in vitro studies on medicinal plants available in Asian countries, including Aloe vera, Alpinia galanga, Centella asiatica, Andrographis paniculata, Curcuma longa, Morinda citrifolia, Trigonella foenum-graecum, Zingiber officinale, Pereskia bleo and Typhonium flagelliforme that have exhibited potential therapeutic properties against breast cancer. The mechanisms of action and potential drug-natural product interactions are discussed because these plants are commonly used in Asian populations. Clinical trials are warranted to further explore the safety and efficacy of these plants to better manage breast cancer in the future.

4 citations

Journal ArticleDOI
TL;DR: It was concluded that rodent tuber Extract ( Typhonium flagelliforme Lodd) can reduce the expression of telomerase in Raji cells, so that the rodent tuber extract has potential as an anticancer through theexpression of telomersase.
Abstract: Cancer cells have a relatively high telomerase activity compared to normal cells, so the cancer cells have the ability to continue to proliferate and undergo mitosis uncontrolled. Telomerase is an enzyme which responsible for telomere length, a DNA segment that is at the end of eukaryotic cell chromosomes. Telomeres and telomerase play a role in the incidence of carcinogenesis. Natural materials such as taro mice ( Typhonium flagelliforme ) have potential as anticancer. The purpose of the study was to determine the effects of plant extracts of rat taro on the expression of telomerase in cancer cell Raji. The research method is experimental studies in some form cancerous cell culture cell line, Raji. Used as a control normal cells is Vero cell. The Culture medium used RPMI for Raji cell and M199 for Vero cell. The study consisted of three groups, control, doses of 1 ½ IC50 and IC50 doses. Expression of telomerase enzyme was measured by the Immunohystochemistry method (IHC). The results showed that the expression of telomerase in cancer cells showed values significantly higher than the normal cells (Vero). Giving mice taro plant extracts ( Typhonium flagelliforme ) were able to decrease the expression of telomerase significantly in both treatment doses. It was concluded that rodent tuber extract ( Typhonium flagelliforme Lodd) can reduce the expression of telomerase in Raji cells, so that the rodent tuber extract ( Typhonium flagelliforme Lodd) has potential as an anticancer through the expression of telomerase. Keywords : telomerase, IHC, Typhonium flagelliforme

4 citations

Journal ArticleDOI
10 May 2021
TL;DR: Empiric treatment with medicinal plants is particularly a concern in cancer treatment, where treatment is currently done with chemotherapy, radiotherapy, and surgery, so design and development of anti-cancer drug candidate from herbal plants are increasingly in demand.
Abstract: Herbal medicines have long been used for traditional treatment of diseases. Our ancestors used plants empirically as treatment and for the maintenance of health inherited from generation to generation. At present, most of the research and development of drugs is still focused on a single compound as the main compound against the treatment target. It is difficult to get a single compound chemical with high selectivity and potential but low toxicity to the target of the disease. Empiric treatment with medicinal plants is particularly a concern in cancer treatment, where treatment is currently done with chemotherapy, radiotherapy, and surgery. The active compound of herbal plants is one alternative in searching for a new anti-cancer because it is believed to have minimal side effects. Therefore, the design and development of anti-cancer drug candidate from herbal plants are increasingly in demand.

3 citations