Fawcett Dw

Bio: Fawcett Dw is an academic researcher. The author has contributed to research in topics: Theileria parva & Apicomplexa. The author has an hindex of 1, co-authored 1 publications receiving 101 citations.

Cytoskeleton of Apicomplexan Parasites

Abstract: The Apicomplexa are a phylum of diverse obligate intracellular parasites including Plasmodium spp., the cause of malaria; Toxoplasma gondii and Cryptosporidium parvum, opportunistic pathogens of immunocompromised individuals; and Eimeria spp. and Theileria spp., parasites of considerable agricultural importance. These protozoan parasites share distinctive morphological features, cytoskeletal organization, and modes of replication, motility, and invasion. This review summarizes our current understanding of the cytoskeletal elements, the properties of cytoskeletal proteins, and the role of the cytoskeleton in polarity, motility, invasion, and replication. We discuss the unusual properties of actin and myosin in the Apicomplexa, the highly stereotyped microtubule populations in apicomplexans, and a network of recently discovered novel intermediate filament-like elements in these parasites.

The piroplasms: life cycle and sexual stages.

Abstract: Publisher Summary The piroplasms are protozoa that are highly pathogenic to cattle, sheep, goats, and occasionally even to man. They comprise two genera—namely, Theileria and Babesia . The diseases they induce, known collectively as “theilerioses” and “babesioses,” cause fevers and lead to important economic losses in the tropics, subtropics, and southern Europe. This chapter highlights the differences between the life cycles of Babesia and Theileria species with respect to their morphology, studied by means of light and electron microscopy. The chapter describes the life cycle of piroplasms. They have a typical sporozoan life cycle comprising three phases: (1) Schizogony, an asexual reproduction phase in the vertebrate host. (2) Gumogony, the formation and fusion of gametes inside the intestinal cells of ixodid ticks. (3) Sporogony, an asexual reproduction in the salivary gland of the tick leading to the infectious, saliva-transmitted sporozoites. Comparative biological and morphological studies show that the economically important piroplasms comprise three groups: (1) Babesia species sensu strictu ; (2) Bubesia equi , B. microti ; and (3) Theileria species.

Phylogeny and evolution of the piroplasms

Abstract: Small subunit ribosomal RNA (srRNA) genes of three Theileria species, one Cytauxzoon and four Babesia species were amplified using the polymerase chain reaction (PCR), cloned and sequenced. Our sequences were aligned with srRNA sequences previously published for eight species of Apicomplexa, one ciliate and one dinoflagellate, the last two being included as free-living outgroup species. Phylogenetic relationships between the organisms were inferred by four in-dependent methods of phylogenetic tree construction using the ciliate Oxytricha nova to root the trees. Our trees fail to show a consensus branching order. They do, however, clearly indicate that the theilerias form a monophyletic taxon derived from a paraphyletic group which includes the species B. equi, C. felis and B. rodhaini. The distance trees indicate that the babesias sensu stricto (B. canis, B. caballi, B. bigemina and B. bovis) form another monophyletic taxon which diverged before the theilerias separated from the above-mentioned paraphyletic group. The parsimony and maximum likelihood trees suggest that the babesias and theilerias are sister taxa, both of which were derived from the paraphyletic group.

Transformation of Leukocytes by Theileria parva and T. annulata

Abstract: Theileria parva and T. annulata provide intriguing models for the study of parasite-host interactions. Both parasites possess the unique property of being able to transform the cells they infect; T. parva transforms T and B cells, whereas T. annulata affects B cells and monocytes/macrophages. Parasitized cells do not require antigenic stimulation or exogenous growth factors and acquire the ability to proliferate continuously. In vivo, parasitized cells undergo clonal expansion and infiltrate both lymphoid and non-lymphoid tissues of the infected host. Theileria-induced transformation is entirely reversible and is accompanied by the expression of a wide range of different lymphokines and cytokines, some of which may contribute to proliferation or may enhance spread and survival of the parasitized cell in the host. The presence of the parasite in the host-cell cytoplasm modulates the state of activation of a number of signal transduction pathways. This, in turn, leads to the activation of transcription factors, including nuclear factor-kappa B, which appear to be essential for the survival of Theileria-transformed T cells.