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TL;DR: This work provides evidence that one mechanism responsible for TAR is RNAP‐mediated replication impairment, and using novel plasmid‐borne recombination constructs in Saccharomyces cerevisiae shows that RNA polymerase II (RNAPII) transcription induces recombination by impairing replication fork progression.

TL;DR: Evidence that deficient mRNP biogenesis causes slowdown or pausing of the replication fork in hpr1Δ mutants is provided and transcription-dependent genomic instability in THO mutants with impaired replication fork progression is linked, suggesting a molecular basis for a connection between inefficient mR NP biogenesis and genetic instability.

TL;DR: It is proposed that deletions can occur as three types of recombination events: the conservative RAD52-dependent reciprocal exchange and the nonconservative events, one-ended invasion crossover, and single-strand annealing (SSA).

TL;DR: It is demonstrated that Hpr1 is a factor required for transcription of long as well as G+C-rich DNA sequences, and both length and G-C content are important elements influencing transcription in vivo.

TL;DR: The results support a stepwise mechanism for the replacement of H2A with Htz1 and demonstrate that a tight control of this mechanism is essential to regulate chromatin dynamics but also to prevent the deleterious consequences of an incomplete nucleosome remodelling.