Felix Wróblewski

Bio: Felix Wróblewski is an academic researcher from Kettering University. The author has contributed to research in topics: Transaminase & Cancer. The author has an hindex of 32, co-authored 48 publications receiving 7770 citations. Previous affiliations of Felix Wróblewski include Ontario Agricultural College.

Lactic dehydrogenase activity in blood.

Abstract: Summary and Conclusions1. Lactic dehydrogenase activity is present in the venous serum of normal human adults. Normal activity ranges from 260 to 850 units per ml with a mean value of470 ± 130 units per ml. 2. Venous whole blood hemolysates of normal adults have a lactic dehydrogenase activity varying between 16,000 to 67,000 units per ml with a mean value of 34,000 ± 12,000 units per ml. 3. Alterations in serum lactic dehydrogenase have been studied in a selected group of disease states. 4. Experimental and clinical myocardial infarction are associated with a rise in serum lactic dehydrogenase activity. 5. Lactic dehydrogenase like serum glutamic oxaloacetic transaminase rises in a characteristic fashion following myocardial infarction.

Transaminase activity in human blood

Abstract: Enzymatic transamination consists of the enzyme catalyzed reversible transfer of the alpha amino nitrogen of an amino acid to an alpha-keto acid with the synthesis of a second amino acid and a second alpha-keto acid. Enzymes catalyzing different transamination reactions are found widely distributed in animal tissues and have been shown to change in activity in some tissues during disease (1-3). These observations prompted the present study to determine if transaminase activity could be demonstrated in human serum and blood cellular elements and, if so, to study any variations in activity of this enzyme in the blood of normal and diseased man.

Serum glutamic pyruvic transaminase in cardiac with hepatic disease.

Abstract: SummaryThe measurement of SGP-T alterations has been found to be a useful tool in the diagnosis and study of acute hepatic disease and appears to be more sensitive than SGO-T in depicting acute hep...

Serum glutamic oxalacetic transaminase activity as an index of liver cell injury: a preliminary report

Abstract: Excerpt INTRODUCTION Glutamic oxalacetic transaminase is widely distributed in animal tissues. Its greatest concentration, however, is in heart muscle, skeletal muscle, brain, liver and kidney, in ...

HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS: A Biochemical and Fine Structural Study

Abstract: A new technique employing continuous recirculating perfusion of the rat liver in situ, shaking of the liver in buffer in vitro, and filtration of the tissue through nylon mesh, results in the conversion of about 50% of the liver into intact, isolated parenchymal cells. The perfusion media consist of: (a) calcium-free Hanks' solution containing 0.05% collagenase and 0.10% hyaluronidase, and (b) magnesium and calcium-free Hanks' solution containing 2 mM ethylenediaminetetraacetate. Biochemical and morphologic studies indicate that the isolated cells are viable. They respire in a medium containing calcium ions, synthesize glucose from lactate, are impermeable to inulin, do not stain with trypan blue, and retain their structural integrity. Electron microscopy of biopsies taken during and after perfusion reveals that desmosomes are quickly cleaved. Hemidesmosome-containing areas of the cell membrane invaginate and appear to pinch off and migrate centrally. Tight and gap junctions, however, persist on the intact, isolated cells, retaining small segments of cytoplasm from formerly apposing parenchymal cells. Cells which do not retain tight and gap junctions display swelling of Golgi vacuoles and vacuoles in the peripheral cytoplasm. Cytoplasmic vacuolization in a small percentage of cells and potassium loss are the only indications of cell injury detected. By other parameters measured, the isolated cells are comparable to normal hepatic parenchymal cells in situ in appearance and function.

Free DNA in the Serum of Cancer Patients and the Effect of Therapy

Abstract: A radioimmunoassay for ng quantities of DNA was developed. [125l]lododeoxyuridine-labeled DNA was used as the antigen, and the serum of a lupus erythematosus patient served as the source of antibody. The level of free DNA in the serum of 173 patients with various types of cancer and in 55 healthy individuals was determined by this radioimmunoassay. DNA concentration in the normal controls had a range of 0 to 100 ng/ml with a mean of 13 +/- 3 ng/ml (S.E.). For comparison purposes, the range of 0 to 50 ng/ml was designated as normal, and 93% of controls were found in this range. In the cancer patients, the DNA concentration ranged from zero to mug levels with a mean of 180 +/- 38 ng/ml. Fifty % of the patients values were found in the range of 0 to 50 ng/ml; the other 50% were between 50 and 5000 ng/ml. No correlation could be seen between DNA levels and the size or location of the primary tumor. Significantly higher DNA levels, however, were found in the serum of patients with metastatic disease (mean of 209 +/- 39 ng/ml), as compared to nonmetastatic patients (mean 100 +/- 30, p less than 0.02). After radiation therapy in lymphoma, lung, ovary, uterus, and cervical tumors, the levels decreased in 66 to 90% of the patients, whereas in glioma, breast, colon, and rectal tumors, the DNA levels decreased only in 16 to 33% of the patients. Generally, the decrease in DNA concene of tumor size and reduction of pain. Conversely, when DNA levels either increased or remained unchanged, a lack of response to the treatment was noted. Of 17 patients who died within a year, 13 showed DNA levels that remained high or unchanged, whereas only 4 showed lower levels during treatment. Persistent high or increasing DNA levels in the circulation, therefore, may signal a relapse and are probably a poor prognostic sign. The relatively high percentage (50%) of cancer patients with apparently normal DNA levels would suggest that this test may have low diagnostic value. It should be pointed out, however, that all these patients represent a selected group considered for radiation therapy, usually after surgery and/or chemotherapy. It is possible that a better correlation between DNA levels and cancer will be obtained prior to the initiation of treatment. On the other hand, DNA in the serum may be an important tool for the evaluation of therapy or the comparison of different regimens.

Pleural Effusions: The Diagnostic Separation of Transudates and Exudates

Abstract: In this prospective study of 150 pleural effusions, the utility of pleural-fluid cell counts, protein levels, and lactic dehydrogenase (LDH) levels for the separation of transudates from e...

Liver enzyme alteration: a guide for clinicians

Abstract: ISOLATED ALTERATIONS OF BIOCHEMICAL MARKERS OF LIVER DAMAGE in a seemingly healthy patient can present a challenge for the clinician. In this review we provide a guide to interpreting alterations to liver enzyme levels. The functional anatomy of the liver and pathophysiology of liver enzyme alteration are briefly reviewed. Using a schematic approach that classifies enzyme alterations as predominantly hepatocellular or predominantly cholestatic, we review abnormal enzymatic activity within the 2 subgroups, the most common causes of enzyme alteration and suggested initial investigations.