Fenglei Jiang

TL;DR: It is shown that membrane insertion of two Sec-independent proteins requires YidC, which is essential for E. coli viability and homologues are present in mitochondria and chloroplasts.

TL;DR: YidC is involved in the translocation but not in the targeting of the Procoat protein, because the protein was partitioned into the membrane in the absence of YidC, and studies are consistent with the understanding that YdC cooperates with the Sec translocase for membrane translocation and that YIDC is required for clearing the protein-conducting channel.

TL;DR: It is reported that the Sec-independent Pf3 coat protein requires the YidC protein specifically for the membrane translocation step, and the role of YIDC is at the stage of folding the Pf3 protein into a transmembrane configuration.

TL;DR: It is proposed that the five C-terminal transmembrane segments of YidC function as a platform for the translocating substrate protein to support its insertion into the membrane.

TL;DR: The role of YidC in the membrane insertion pathway will be reviewed, which directly interacts with membrane proteins during the membrane protein insertion process and assists in the folding of the hydrophobic regions into the membrane bilayer.