scispace - formally typeset
Search or ask a question
Author

Fengqing Gai

Bio: Fengqing Gai is an academic researcher from University of Jinan. The author has contributed to research in topics: Fluorescence & Chemistry. The author has an hindex of 1, co-authored 5 publications receiving 4 citations.
Topics: Fluorescence, Chemistry, Lysosome, Autophagy, Medicine

Papers
More filters
Journal ArticleDOI
01 Apr 2021-Talanta
TL;DR: The capacity of TDCQ for facilitating the in-depth study of LDs and applying to the identification of cancer cells is demonstrated, demonstrating outstanding photostability and low toxicity.

22 citations

Journal ArticleDOI
TL;DR: In this article, a "turn-off" visual and near infrared (NIR) fluorescent dual emission probe D6 based on polyhedral oligomeric silsesquioxanes (POSS) was successfully designed and synthesized.
Abstract: Hypochlorous acid (HClO) is closely related to many diseases and is an inevitable part of the physiological processes. It is significant to detect HClO in mitochondria for getting meaningful physiological and pathological information. However, adequate tools to detect HClO with emissions in two channels are rarely reported. To achieve this target, in this work, a "turn-off" visual and near infrared (NIR) fluorescent dual emission probe D6 based on polyhedral oligomeric silsesquioxanes (POSS) was successfully designed and synthesized. D6 showed high selectivity and sensitivity to HClO. Notably, the emission wavelength of D6 reached 820 nm due to the assistance of the POSS cage. In addition, bioimaging experiments clearly showed that probe D6 promoted the visualization of exogenous and endogenous HClO in living HepG2 cells and zebrafish models.

6 citations

Journal ArticleDOI
TL;DR: In this article, a Si-O bond was added to the Si-Si-O probe and the probe exhibited a broad transparency in the visible light range and had excellent photostability.
Abstract: As an essential gasotransmitter, carbon monoxide (CO) had gradually become a research hotspot in that it possessed important physiological functions and unique pharmacological properties. However, to date, no report has focused on the topic of detecting CO both in vivo and using films. To open up a new field of CO probes, for the first time, we designed a probe (PMAH-CO) that showed a distinctive ratio emission characteristic and displayed the quantitative distribution of CO in HeLa cells and zebrafish with a higher signal-to-noise ratio. Meanwhile, the fluorescent polysiloxane-based film (PMF) containing PMAH-CO exhibited an excellent response to CO. Due to the addition of the Si-O bond, the probe exhibited a broad transparency in the visible light range and had excellent photostability. Moreover, the probe was economically viable, easy to handle, and suitable for biological research. Hence, PMAH-CO and PMF would open up the road to broaden the application of silicone materials in the field of fluorescence imaging.

4 citations

Journal ArticleDOI
TL;DR: In this paper, a polysiloxane-based probe (PA-1) was used to visualize the autophagy process in a lysosome and pH-responsive manner.
Abstract: Autophagy is an essential process for the degradation of malfunctional organelles and the response to cancer immunotherapy. The occurrence of defective autophagy is correlated to obesity, aging, neurological diseases, inflammation and tumors. Therefore, it is urgent to accurately depict the autophagic process. We herein architecture a polysiloxane-based probe (PA-1) that was lysosome targeted and pH-responsive for discerning autophagy process. We have demonstrated that PA-1 enabled specific visualization of lysosomal autophagy. PA-1 also detected the down-regulation of the pH value that accompanied the autophagy process under starvation stimulation. During autophagy, the green fluorescence of some spots in the lysosomes gradually decreased along with the emergence of strong agglomerated red fluorescence, which might be caused by the membrane fusion of lysosomes and autophagosomes. The location of the autolysosome could be precisely displayed by the ratio image. In addition, we observed that the autophagy inhibitor CQ could increase the pH of the intracellular environment and inhibit autophagy. The design strategy will broaden the research field of polysiloxane in cellular physiological processes and serves as a candidate for basic research on autophagic processes and effective therapeutic strategies for autophagy-related diseases.

4 citations

Journal ArticleDOI
TL;DR: Two novel multistimuli-responsive fluorescent probes with lysosomal targeting in dual channels based on spiropyrans were rationally designed and synthesized and their potential applications in the field of dual-anticounterfeiting were explored based on their photoluminescence ability.
Abstract: Lysosomes, as the main degradative organelles, play an important role in a variety of cellular metabolic activities including autophagy and apoptosis, catabolism and transporting substances. Lysosomal autophagy is an important physiological process and causes a slight change in the intra-lysosomal pH to facilitate the breakdown of macromolecular proteins. Therefore, detecting the fluctuation of intra-lysosomal pH is of great significance in monitoring physiological and pathological activities in living organisms. However, few probes have enabled the ratiometric monitoring of lysosomal pH and lysosomal autophagy in dual channels. Fortunately, spiropyrans, as compounds with multistimuli-responsive discoloration properties, form two different isomers under acid induction and ultraviolet induction. To fill this gap, in this work, two novel multistimuli-responsive fluorescent probes with lysosomal targeting in dual channels based on spiropyrans were rationally designed and synthesized. Notably, the two probes exhibited different absorption wavelengths in their UV-responsive and pH-responsive moieties due to their different electron-donating groups. Moreover, bioimaging experiments clearly demonstrate that the probes Lyso-SP and Lyso-SQ monitor lysosomal autophagy by facilitating the visualization of fluctuations in intra-lysosomal pH. Meanwhile, their potential applications in the field of dual-anticounterfeiting were explored based on their photoluminescence ability. We expect that more multistimuli-responsive fluorescent probes can be developed by this design approach.

3 citations


Cited by
More filters
Journal ArticleDOI
TL;DR: In this article, a review highlights the recent trends in the development of fluorescent organosilicon compounds as powerful tools for bio-imaging within diverse living systems and summarizes the roles of Si O Si bonds acted in bioimaging.

24 citations

Journal ArticleDOI
TL;DR: In this paper , the authors developed a novel fluorescent chemosensor with a hybrid structure of 3-pyridinonitrile-coumarin derivatives by molecular design strategy that can effectively label intracellular lipid droplets.

14 citations

Journal ArticleDOI
TL;DR: The polarity-sensitive emission character of this probe enables us to quantitatively determine the LD polarity via spectral scan imaging and the cancer cells displaying lower polarity of LDs than the normal cells have been systematically demonstrated.
Abstract: Lipid droplets (LDs), which are ubiquitous organelles existing in almost all eukaryotic cells, have attracted a lot of attention in the field of cell biology over the last decade. For the biological study of LDs via fluorescence imaging, the superior LD fluorescent probes with environmental polarity-sensitive character are highly desired and powerful but are very scarce. Herein, we have newly developed such a kind of fluorescent probe named LDs-Red which enables us to visualize LDs and to further reveal their polarity information. This fluorescent probe displays the advantages of intense red/near-infrared emission, high LD staining specificity, and good photostability; thus, it would be very useful for LD fluorescence imaging application. As a result, the three-dimensional confocal imaging to visualize spatial distribution of LDs and the multicolor confocal imaging to simultaneously observe LDs and other cellular organelles have been realized using this new LD fluorescent probe. Furthermore, the polarity-sensitive emission character of this probe enables us to quantitatively determine the LD polarity via spectral scan imaging. Consequently, the cancer cells (HepG2, HeLa, and Panc02) displaying lower polarity of LDs than the normal cells (L929, U251, and HT22) have been systematically demonstrated. In addition, this polarity-sensitive probe displaying shorter fluorescence wavelengths in cancer cells than in normal cells has an important and potential ability to distinguish them.

13 citations

Journal ArticleDOI
TL;DR: In this paper , the first naphthalimide-triphenylamine-based dual-site fluorescent probe NATPA was developed for simultaneously imaging intracellular H2O2 and polarity fluctuations.
Abstract: H2O2 and polarity are quite important in many physiological and pathological processes, and their relationship is complicated and obscure for researchers. Thus, it is vital and challenging to achieve simultaneous detection of H2O2 and polarity in vivo. Herein, the first naphthalimide-triphenylamine-based dual-site fluorescent probe NATPA is developed for simultaneously imaging intracellular H2O2 and polarity fluctuations. It exhibits excellent sensitivity (LOD = 44 nM), selectivity, and fast response (15 min) to H2O2 and a superior capacity for detecting polarity upon the intramolecular charge transfer (ICT) effect. Besides, the probe displays low cytotoxicity and lipid droplet targeting and is further applied in imaging H2O2 and polarity fluctuations in HepG2 and L-02 cells, so that NATPA is qualified to distinguish cancer cells from normal cells. This research contributes a new design principle for the construction of dual-site fluorescent probes for simultaneously detecting active molecules and polarity.

10 citations

Journal ArticleDOI
TL;DR: In this article , the healing process of internal and external microcracks was visualized and quantified with laser scanning confocal microscope (LSCM) and step profiler.

9 citations