F
Florian Gnad
Researcher at Genentech
Publications - 70
Citations - 19908
Florian Gnad is an academic researcher from Genentech. The author has contributed to research in topics: Phosphorylation & Proteomics. The author has an hindex of 45, co-authored 69 publications receiving 17870 citations. Previous affiliations of Florian Gnad include Cell Signaling Technology & Autonomous University of Madrid.
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Journal ArticleDOI
Lysine Acetylation Targets Protein Complexes and Co-Regulates Major Cellular Functions
Chunaram Choudhary,Chanchal Kumar,Florian Gnad,Michael L. Nielsen,Michael Rehman,Tobias C. Walther,Jesper V. Olsen,Matthias Mann +7 more
TL;DR: A proteomic-scale analysis of protein acetylation suggests that it is an important biological regulatory mechanism and the regulatory scope of lysine acetylations is broad and comparable with that of other major posttranslational modifications.
Journal ArticleDOI
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
Jesper V. Olsen,Blagoy Blagoev,Florian Gnad,Boris Macek,Boris Macek,Chanchal Kumar,Peter Mortensen,Matthias Mann +7 more
TL;DR: A general mass spectrometric technology is developed and applied for identification and quantitation of phosphorylation sites as a function of stimulus, time, and subcellular location to provide a missing link in a global, integrative view of cellular regulation.
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Quantitative Phosphoproteomics Reveals Widespread Full Phosphorylation Site Occupancy During Mitosis
Jesper V. Olsen,Michiel Vermeulen,Anna Santamaria,Chanchal Kumar,Martin L. Miller,Martin L. Miller,Lars Juhl Jensen,Florian Gnad,Jürgen Cox,Thomas Skøt Jensen,Erich A. Nigg,Søren Brunak,Søren Brunak,Matthias Mann +13 more
TL;DR: High-resolution mass spectrometry–based proteomics was applied to investigate the proteome and phosphoproteome of the human cell cycle on a global scale and quantified 6027 proteins and 20,443 unique phosphorylation sites and their dynamics, finding that nuclear proteins and proteins involved in regulating metabolic processes have high phosphorylated site occupancy in mitosis, suggesting that these proteins may be inactivated by phosphorylate in mitotic cells.
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Recurrent R-spondin fusions in colon cancer
Somasekar Seshagiri,Eric Stawiski,Steffen Durinck,Zora Modrusan,Elaine E. Storm,Caitlin B. Conboy,Subhra Chaudhuri,Yinghui Guan,Vasantharajan Janakiraman,Bijay S. Jaiswal,Joseph Guillory,Connie Ha,Gerrit J. P. Dijkgraaf,Jeremy Stinson,Florian Gnad,Melanie A. Huntley,Jeremiah D. Degenhardt,Peter M. Haverty,Richard Bourgon,Weiru Wang,Hartmut Koeppen,Robert Gentleman,Timothy K. Starr,Zemin Zhang,David A. Largaespada,Thomas D. Wu,Frederic J. de Sauvage +26 more
TL;DR: The R-spondin gene fusions, several new recurrent mutations in the Wnt pathway gene TCF7L2, chromatin-remodelling genes such as TET2 and TET3 and receptor tyrosine kinases including ERBB3 are identified and shown to be capable of potentiating Wnt signalling.
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Comprehensive genomic analysis identifies SOX2 as a frequently amplified gene in small-cell lung cancer
Charles M. Rudin,Steffen Durinck,Eric Stawiski,John T. Poirier,Zora Modrusan,David S. Shames,Emily Bergbower,Yinghui Guan,James Shin,Joseph Guillory,Celina Sanchez Rivers,Catherine K. Foo,Deepali Bhatt,Jeremy Stinson,Florian Gnad,Peter M. Haverty,Robert Gentleman,Subhra Chaudhuri,Vasantharajan Janakiraman,Bijay S. Jaiswal,Chaitali Parikh,Wenlin Yuan,Zemin Zhang,Hartmut Koeppen,Thomas D. Wu,Howard M. Stern,Robert L. Yauch,Kenneth E. Huffman,Diego D Paskulin,Peter B. Illei,Marileila Varella-Garcia,Adi F. Gazdar,Frederic J. de Sauvage,Richard Bourgon,John D. Minna,Malcolm V. Brock,Somasekar Seshagiri +36 more
TL;DR: Exome, transcriptome and copy-number alteration data are obtained from approximately 53 samples consisting of 36 primary human SCLC and normal tissue pairs and 17 matched SCLCs and lymphoblastoid cell lines to identify several potential targets for therapeutic intervention.