F
Florian M. Wurm
Researcher at École Polytechnique Fédérale de Lausanne
Publications - 264
Citations - 14581
Florian M. Wurm is an academic researcher from École Polytechnique Fédérale de Lausanne. The author has contributed to research in topics: Chinese hamster ovary cell & Transfection. The author has an hindex of 58, co-authored 260 publications receiving 13787 citations. Previous affiliations of Florian M. Wurm include Lawrence Livermore National Laboratory & Ludwig Institute for Cancer Research.
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Production of recombinant protein therapeutics in cultivated mammalian cells
TL;DR: Recently, the productivity of mammalian cells cultivated in bioreactors has reached the gram per liter range in a number of cases, a more than 100-fold yield improvement over titers seen for similar processes in the mid-1980s.
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Designing CD4 immunoadhesins for AIDS therapy
Daniel J. Capon,Steven M. Chamow,Joyce Mordenti,Scot A. Marsters,Timothy J. Gregory,Hiroaki Mitsuya,Randal A. Byrn,Catherine Lucas,Florian M. Wurm,Jerome E. Groopman,Samuel Broder,Douglas H. Smith +11 more
TL;DR: A newly-constructed antibody-like molecule containing the gp!20-binding domain of the receptor for human immunodeficiency virus blocks HIV-1 infection of T cells and monocytes, making it a good candidate for therapeutic use.
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Transfecting Mammalian Cells: Optimization of Critical Parameters Affecting Calcium-Phosphate Precipitate Formation
TL;DR: Transient expression levels 2 days after DNA transfer and titers obtained from stable cell lines, emerging weeks later, showed strong correlation and better reproducibility and higher efficiencies both for transient and for stable transfections were gained.
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Protection of chimpanzees from infection by HIV-1 after vaccination with recombinant glycoprotein gp120 but not gp160
Phillip W. Berman,Tim Gregory,Lavon Riddle,Gerald R. Nakamura,Mark Champe,James P. Porter,Florian M. Wurm,Robert D. Hershberg,Cobb Ek,Jorg W. Eichberg +9 more
TL;DR: It is demonstrated that recombinant gp120, formulated in an adjuvant approved for human use, can elicit protective immunity against a homologous strain of HIV-1.
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Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives.
TL;DR: This article summarizes efforts in industry and academia to use TGE to produce tens to hundreds of milligrams of r-proteins for either fundamental research or preclinical studies.