F
Francisco Fierro
Researcher at Universidad Autónoma Metropolitana
Publications - 57
Citations - 1804
Francisco Fierro is an academic researcher from Universidad Autónoma Metropolitana. The author has contributed to research in topics: Penicillium chrysogenum & Gene. The author has an hindex of 23, co-authored 55 publications receiving 1598 citations. Previous affiliations of Francisco Fierro include University of León.
Papers
More filters
Journal ArticleDOI
The penicillin gene cluster is amplified in tandem repeats linked by conserved hexanucleotide sequences
Francisco Fierro,José Luis Barredo,Bruno Díez,Santiago Gutiérrez,Francisco J. Fernández,Juan F. Martin +5 more
TL;DR: The penicillin biosynthetic genes of Penicillium chrysogenum AS-P-78 were located in a 106.5-kb DNA region that is amplified in tandem repeats (five or six copies) linked by conserved TTTACA sequences, which appear to arise by mutation-induced site-specific recombination at the conserved hexanucleotide sequences.
Journal ArticleDOI
Mutants blocked in penicillin biosynthesis show a deletion of the entire penicillin gene cluster at a specific site within a conserved hexanucleotide sequence
TL;DR: The frequent deletion of the pen gene cluster at this point may indicate that this cluster is located in an unstable genetic region, flanked by hot spots of recombination, that is easily lost by mutagen-induced recombination.
Journal ArticleDOI
Resolution of four large chromosomes in penicillin-producing filamentous fungi: the penicillin gene cluster is located on chromosome II (9.6 Mb) in Penicillium notatum and chromosome I (10.4 Mb) in Penicillium chrysogenum.
TL;DR: A major chromosomal rearrangement seems to have occurred in this high penicillin producing strain of Penicillium chrysogenum and a fast moving DNA band observed in all gels corresponds to mitochondrial DNA.
Journal ArticleDOI
Filamentous fungi from extreme environments as a promising source of novel bioactive secondary metabolites.
TL;DR: A preliminary analysis of three filamentous fungi genomes found that several of the predicted SM gene clusters are probably involved in the biosynthesis of compounds not yet described, a promising path to discover new natural compounds as a source of medically useful drugs.
Journal ArticleDOI
Production of penicillin by fungi growing on food products: identification of a complete penicillin gene cluster in Penicillium griseofulvum and a truncated cluster in Penicillium verrucosum
TL;DR: A study of the penicillin production ability among fungi of the genus Penicillium that are used as starters for cheese and meat products or that are frequently isolated from food products was described.