Frank Costantini

TL;DR: Here are recorded the tech- niques for preparing, inserting and analysing DNA sequences, for retroviral infection of mice, for production and use of EC and EK cells as vehicles for engineered sequences and for nuclear transplantation - all against a background of the basic procedures required for pro- ducing and handling the em- bryos.

TL;DR: In contrast to existing lacZ reporter lines, where lacZ expression cannot easily be detected in living tissue, the EYFP and ECFP reporter strains are useful for monitoring the expression of Cre and tracing the lineage of these cells and their descendants in cultured embryos or organs.

TL;DR: The results strongly suggest that Axin2 is a direct target of the Wnt pathway, mediated through Tcf/LEF factors, and participates in a negative feedback loop which could serve to limit the duration or intensity of a Wnt-initiated signal.

TL;DR: It is shown that mice homozygous for a targeted mutation in c-ret develop to term, but die soon after birth, showing renal agenesis or severe dysgenesis, and lacking enteric neurons throughout the digestive tract, indicating an essential component of a signalling pathway required for renal organogenesis and enteric neurogenesis.

TL;DR: The disruption of the X-linked GATA-1 gene by homologous recombination in a male (XY) murine embryonic stem cell line and testing the Gata-1-deficient cells for their ability to contribute to different tissues in chimaeric mice demonstrates that GATA, the zinc-finger transcription factor, is required for the normal differentiation of erythroid cells, and that other GATAS cannot compensate for its absence.