F
Fred Christians
Researcher at Pacific Biosciences
Publications - 48
Citations - 7076
Fred Christians is an academic researcher from Pacific Biosciences. The author has contributed to research in topics: Nucleic acid & Polymerase. The author has an hindex of 26, co-authored 48 publications receiving 6669 citations. Previous affiliations of Fred Christians include University of Washington & Stanford University.
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Journal ArticleDOI
Real-Time DNA Sequencing from Single Polymerase Molecules
John Eid,Adrian Fehr,Jeremy Gray,Khai Luong,John Lyle,Geoff Otto,Paul Peluso,David R. Rank,Primo Baybayan,Brad Bettman,Arkadiusz Bibillo,Keith Bjornson,Bidhan Chaudhuri,Fred Christians,Ronald L. Cicero,Sonya Clark,Ravindra V. Dalal,Alex DeWinter,John Dixon,Mathieu Foquet,Alfred Gaertner,Paul Hardenbol,Cheryl Heiner,Kevin Hester,David P. Holden,Gregory J. Kearns,Xiangxu Kong,Ronald Kuse,Yves Lacroix,Steven Lin,Paul Lundquist,Congcong Ma,Patrick Marks,Mark Maxham,Devon Murphy,Insil Park,Thang Pham,Michael Phillips,Joy Roy,Robert Sebra,Gene Shen,Jon M. Sorenson,Austin B. Tomaney,Kevin Travers,Mark Trulson,John Vieceli,Jeffrey Wegener,Dawn Wu,Alicia Yang,Denis Zaccarin,Peter Zhao,Frank Zhong,Jonas Korlach,Stephen Turner +53 more
TL;DR: Single-molecule, real-time sequencing data obtained from a DNA polymerase performing uninterrupted template-directed synthesis using four distinguishable fluorescently labeled deoxyribonucleoside triphosphates (dNTPs) are presented.
Journal ArticleDOI
E2Fs regulate the expression of genes involved in differentiation, development, proliferation, and apoptosis
Heiko Muller,Adrian P. Bracken,Richard Vernell,M. Cristina Moroni,Fred Christians,Emanuela Grassilli,Elena Prosperini,Elena Vigo,Jonathan D. Oliner,Kristian Helin +9 more
TL;DR: It is shown that the E2Fs control the expression of several genes that are involved in cell proliferation and apoptosis, differentiation, and development and provide possible genetic explanations to the variety of phenotypes observed as a consequence of a deregulated pRB/E2F pathway.
Journal ArticleDOI
Induction of gadd45 and jnk/sapk-dependent apoptosis following inducible expression of brca1
D. Paul Harkin,Jonathan F. Bean,David B. Miklos,Young-Han Song,Vivi Truong,Christoph Englert,Fred Christians,Leif W. Ellisen,Shyamala Maheswaran,Jonathan D. Oliner,Daniel A. Haber +10 more
TL;DR: The p53-independent induction of GADD45 by BRCA1 and its activation of JNK/SAPK suggest a pathway for BRC a1-induced apoptosis, a signaling pathway potentially linked to GADD 45 gene family members.
Journal ArticleDOI
Analytical and clinical validation of a microbial cell-free DNA sequencing test for infectious disease.
Timothy A. Blauwkamp,Simone A. Thair,Michael Rosen,Lily Blair,Martin S. Lindner,Igor D. Vilfan,Trupti Kawli,Fred Christians,Shivkumar Venkatasubrahmanyam,Gregory D. Wall,Anita Cheung,Zoë N. Rogers,Galit Meshulam-Simon,Huijse Liza,Sanjeev Balakrishnan,James V. Quinn,Desiree Hollemon,David K. Hong,Marla Lay Vaughn,Mickey Kertesz,Sivan Bercovici,Judith C. Wilber,Samuel Yang +22 more
TL;DR: Analytical and clinical validation of a next-generation sequencing test that identifies and quantifies microbial cell-free DNA in plasma from 1,250 clinically relevant bacteria, DNA viruses, fungi and eukaryotic parasites is described.
Journal ArticleDOI
Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling
Fred Christians,Fred Christians,Leonardo Scapozza,Andreas Crameri,Gerd Folkers,Willem P. C. Stemmer +5 more
TL;DR: Molecular modeling suggests that the active sites of both evolved enzymes better accommodate the azido group of AZT at the expense of thymidine, which could be useful for anti-HIV gene therapy, and similar directed-evolution approaches could improve other enzyme–prodrug combinations.