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Author

Fred Singer

Bio: Fred Singer is an academic researcher from University of Oregon. The author has contributed to research in topics: Zebrafish & Centromere. The author has an hindex of 3, co-authored 3 publications receiving 1517 citations.

Papers
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Journal ArticleDOI
28 May 1981-Nature
TL;DR: Clones of homozygous fish have been produced from individual homozygotes and associated genetic methods facilitate genetic analyses of this vertebrate.
Abstract: Homozygous diploid zebra fish have been produced on a large scale by the application of simple physical treatments. Clones of homozygous fish have been produced from individual homozygotes. These clones and associated genetic methods will facilitate genetic analyses of this vertebrate.

1,203 citations

Journal ArticleDOI
01 Feb 1986-Genetics
TL;DR: The gol-1, gol-2, alb-1 and spa-1 mutations affect pigment pattern in the zebrafish and it is shown here that these loci are unlinked to each other.
Abstract: The gol-1, gol-2, alb-1 and spa-1 mutations affect pigment pattern in the zebrafish. We show here that these loci are unlinked to each other. In addition, gene-centromere distances were determined for these loci by analysis of half-tetrads obtained by the inhibition of the second meiotic division. The fractions of tetratype (second-division segregation) tetrads range from 0.24 (spa-1) to 0.89 (gol-1). The observation of greater than 0.67 second-division segregation indicates that the zebrafish has high chiasma interference.

248 citations

Journal ArticleDOI
01 Jan 1983-Genetics
TL;DR: Specific locus and recessive lethal mutations are induced by γ-rays with approximately first order kinetics in the zebrafish and the surprisingly low ratio (100:1) of recessive lethals to specific locus mutations may be due to the induction of large deficiencies by ε-rays.
Abstract: Specific locus and recessive lethal mutations are induced by gamma-rays with approximately first order kinetics in the zebrafish (Brachydanio rerio) with frequencies of 4 x 10(-5) r(-1 ) and 4 x 10(-3) r(-1), respectively. The surprisingly low ratio (100:1) of recessive lethals to specific locus mutations may be due to the induction of large deficiencies by gamma-rays.

146 citations


Cited by
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Journal ArticleDOI
TL;DR: A series of stages for development of the embryo of the zebrafish, Danio (Brachydanio) rerio is described, providing for flexibility and continued evolution of the staging series as the authors learn more about development in this species.
Abstract: We describe a series of stages for development of the embryo of the zebrafish, Danio (Brachydanio) rerio. We define seven broad periods of embryogenesis--the zygote, cleavage, blastula, gastrula, segmentation, pharyngula, and hatching periods. These divisions highlight the changing spectrum of major developmental processes that occur during the first 3 days after fertilization, and we review some of what is known about morphogenesis and other significant events that occur during each of the periods. Stages subdivide the periods. Stages are named, not numbered as in most other series, providing for flexibility and continued evolution of the staging series as we learn more about development in this species. The stages, and their names, are based on morphological features, generally readily identified by examination of the live embryo with the dissecting stereomicroscope. The descriptions also fully utilize the optical transparancy of the live embryo, which provides for visibility of even very deep structures when the embryo is examined with the compound microscope and Nomarski interference contrast illumination. Photomicrographs and composite camera lucida line drawings characterize the stages pictorially. Other figures chart the development of distinctive characters used as staging aid signposts.

10,612 citations

Journal ArticleDOI
Kerstin Howe, Matthew D. Clark, Carlos Torroja1, Carlos Torroja2  +171 moreInstitutions (11)
25 Apr 2013-Nature
TL;DR: A high-quality sequence assembly of the zebrafish genome is generated, made up of an overlapping set of completely sequenced large-insert clones that were ordered and oriented using a high-resolution high-density meiotic map, providing a clearer understanding of key genomic features such as a unique repeat content, a scarcity of pseudogenes, an enrichment of zebra fish-specific genes on chromosome 4 and chromosomal regions that influence sex determination.
Abstract: Zebrafish have become a popular organism for the study of vertebrate gene function. The virtually transparent embryos of this species, and the ability to accelerate genetic studies by gene knockdown or overexpression, have led to the widespread use of zebrafish in the detailed investigation of vertebrate gene function and increasingly, the study of human genetic disease. However, for effective modelling of human genetic disease it is important to understand the extent to which zebrafish genes and gene structures are related to orthologous human genes. To examine this, we generated a high-quality sequence assembly of the zebrafish genome, made up of an overlapping set of completely sequenced large-insert clones that were ordered and oriented using a high-resolution high-density meiotic map. Detailed automatic and manual annotation provides evidence of more than 26,000 protein-coding genes, the largest gene set of any vertebrate so far sequenced. Comparison to the human reference genome shows that approximately 70% of human genes have at least one obvious zebrafish orthologue. In addition, the high quality of this genome assembly provides a clearer understanding of key genomic features such as a unique repeat content, a scarcity of pseudogenes, an enrichment of zebrafish-specific genes on chromosome 4 and chromosomal regions that influence sex determination.

3,573 citations

Journal ArticleDOI
TL;DR: The lability of sex-determination systems in fish makes some species sensitive to environmental pollutants capable of mimicking or disrupting sex hormone actions, and such observations provide important insight into potential impacts from endocrine disruptors, and can provide useful monitoring tools for impacts on aquatic environments.

2,283 citations

Journal ArticleDOI
TL;DR: This Review surveys the achievements and potential of zebrafish for modelling human diseases and for drug discovery and development.
Abstract: Despite the pre-eminence of the mouse in modelling human disease, several aspects of murine biology limit its routine use in large-scale genetic and therapeutic screening. Many researchers who are interested in an embryologically and genetically tractable disease model have now turned to zebrafish. Zebrafish biology allows ready access to all developmental stages, and the optical clarity of embryos and larvae allow real-time imaging of developing pathologies. Sophisticated mutagenesis and screening strategies on a large scale, and with an economy that is not possible in other vertebrate systems, have generated zebrafish models of a wide variety of human diseases. This Review surveys the achievements and potential of zebrafish for modelling human diseases and for drug discovery and development.

1,998 citations

Journal ArticleDOI
TL;DR: It is found that the zebrafish fli1 promoter is able to drive expression of enhanced green fluorescent protein (EGFP) in all blood vessels throughout embryogenesis, and these transgenic lines allow detailed analysis of both wild type and mutant embryonic vasculature.

1,939 citations