F
Fuguo Jiang
Researcher at University of California, Berkeley
Publications - 25
Citations - 6092
Fuguo Jiang is an academic researcher from University of California, Berkeley. The author has contributed to research in topics: RNA & Cas9. The author has an hindex of 19, co-authored 25 publications receiving 4764 citations. Previous affiliations of Fuguo Jiang include California Institute for Quantitative Biosciences & Center for Advanced Biotechnology and Medicine.
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Journal ArticleDOI
CRISPR-Cas9 Structures and Mechanisms.
Fuguo Jiang,Jennifer A. Doudna +1 more
TL;DR: This review aims to provide an in-depth mechanistic and structural understanding of Cas9-mediated RNA-guided DNA targeting and cleavage and provides a framework for rational engineering aimed at altering catalytic function, guide RNA specificity, and PAM requirements and reducing off-target activity for the development of Cas 9-based therapies against genetic diseases.
Journal ArticleDOI
Structures of Cas9 Endonucleases Reveal RNA- Mediated Conformational Activation
Martin Jinek,Fuguo Jiang,David W. Taylor,David W. Taylor,Samuel H. Sternberg,Emine Kaya,Enbo Ma,Carolin Anders,Michael H. Hauer,Kaihong Zhou,Steven Lin,Matias Kaplan,Anthony T. Iavarone,Emmanuelle Charpentier,Emmanuelle Charpentier,Eva Nogales,Jennifer A. Doudna +16 more
TL;DR: To compare the architectures and domain organization of diverse Cas9 proteins, the atomic structures of Cas9 from Streptococcus pyogenes and Actinomyces naeslundii and AnaCas9 were determined by x-ray crystallography and three-dimensional reconstructions of apo-SpyCas9, SpyCas9:RNA, and SpyCas 9:RNA:DNA were obtained by negative-stain single-particle electron microscopy.
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Innate immunity induced by composition-dependent RIG-I recognition of hepatitis C virus RNA
TL;DR: This work identifies the polyuridine motif of the HCV genome 3′ non-translated region and its replication intermediate as the PAMP substrate of RIG-I, and shows that this and similar homopolyuridine or homopolyriboadenine motifs present in the genomes of RNA viruses are the chief feature of Rig-I recognition and immune triggering in human and murine cells.
Journal ArticleDOI
Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair
Kunwoo Lee,Michael J. Conboy,Hyo Min Park,Fuguo Jiang,Hyun Jin Kim,Hyun Jin Kim,Mark A. DeWitt,Vanessa A Mackley,Kevin Chang,Anirudh Rao,Colin M. Skinner,Tamanna Shobha,Melod Mehdipour,Hui Liu,Wen Chin Huang,Freeman Lan,Nicolas Bray,Song Li,Jacob E. Corn,Kazunori Kataoka,Jennifer A. Doudna,Irina M. Conboy,Niren Murthy +22 more
TL;DR: It is demonstrated that a delivery vehicle composed of gold nanoparticles conjugated to DNA and complexed with cationic endosomal disruptive polymers can deliver Cas9 ribonucleoprotein and donor DNA into a wide variety of cell types and efficiently correct the DNA mutation that causes Duchenne muscular dystrophy in mice via local injection, with minimal off-target DNA damage.
Journal ArticleDOI
Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage.
Fuguo Jiang,David W. Taylor,Janice S. Chen,Jack E. Kornfeld,Kaihong Zhou,Aubri J. Thompson,Eva Nogales,Jennifer A. Doudna +7 more
TL;DR: Molecular structures of the catalytically active Streptococcus pyogenes Cas9 R-loop are determined that show the displaced DNA strand located near the RuvC nuclease domain active site in a conformation essential for concerted DNA cutting.