G. E. Foley

Bio: G. E. Foley is an academic researcher from National Foundation for Cancer Research. The author has contributed to research in topics: Tissue culture & Thymine. The author has an hindex of 5, co-authored 6 publications receiving 134 citations.

Differentiation of normal and neoplastic cells maintained in tissue culture by implantation into normal hamsters.

Abstract: Summary Quantitated inocula of 14 tissue culture cell lines which are remarkably similar in vitro were implanted into cheek pouches of unconditioned Syrian hamsters. All cell lines grew in the cheek pouch when 1 × 106 cells were implanted, but only those cell lines derived from neoplastic tissue produced tumors when inocula contained 1 × 104 cells. Other experiments indicated that this difference in the growth potential of cell lines deriving from neoplastic tissue also may be delineated in hamsters conditioned with cortisone acetate by implantation with 1 × 103 or fewer cells.

Sustained Propagation of Sarcoma 180 in Tissue Culture.

Abstract: Summary1. A strain of Sarcoma 180 maintained by routine trocar implant passage in CFW mice has been isolated and serially propagated in vitro from biopsy specimens in a medium containing only the growth factors demonstrably essential for the HeLa cell and the L strain of mouse fibroblasts. The generation time in these media is circa 30 hrs and this cell line is now in the 32 nd transplant, having undergone more than 100 generations during 230 days in vitro. 2. Inocula prepared from these cultures after 215 days in vitro have produced characteristic sarcomata within 3-5 days in 100% of the CFW mice implanted subcutaneously with 1.0 × 105 or more cells. The ED50 in CFW mice is circa 4.5-5.0 × 103 cells, and the latent period between injection and the development of palpable tumors increases with decreasing inocula.

Growth of human epidermoid carcinomas (strains KB and HeLa) in hamsters from tissue culture inocula.

Abstract: Summary1. The KB and HeLa strains of human epidermoid carcinoma have been established in the cheek pouches of golden hamsters by means of quantitated suspensions prepared from tissue cultures in a medium providing the specific amino acid and vitamin requirements of these cell lines. 2. Strain KB, isolated directly from a biopsy specimen in these media, has been grown in LAFi mice bearing homologous ACTH secreting pituitary tumors. This strain also has been established in the cheek pouch of non-conditioned hamsters but thus far has failed to grow in non-conditioned mice. 3. The use of quantitated tissue culture inocula for the production of tumors in the hamster cheek pouch for chemotherapeutic studies is suggested.

Chemical and Biological Studies on 1,2-Dihydro-s-triazines. IV. Activity in Pteroylglutamic Acid-Streptococcus faecaelis No. 8043 Systems

Abstract: SummaryA series of 71 1,2-dihydro-s-tri-azines have been studied in Streptococcus faecalis No. 8 043-pteroylglutamic acid assay systems. The active derivatives exhibit noncompetitive inhibition over a wide range of concentrations of PGA and differ from 4-aminopteroylglutamic acid in that inhibition is not relieved by adenine or guanine and is irreversible with pteroylglutamic acid. Differences in microbiological activity could be correlated with certain variations in structure and substitution in the molecule with the series. Maximum activity is obtained with 2,2-dimethyl substitution in the triazine ring together with para-and meta-halogen sub-stituents in the phenyl ring. The inhibitory effect of these compounds on Streptococcus faecalis No. 8043 is reversed by appropriate concentrations of dihydropteroylglutamic acid, N10-formylpteroylglutamic acid, synthetic and natural citrovorum factor and thymine, suggesting that these compounds interfere with the conversion of pteroylglutamic acid to citrovorum fa...

Chemical and Biological Studies on 1,2-Dihydro-s-triazines. V. Inhibition of Derivatives of Pteroylglutamic Acid

Abstract: SummaryDihydropteroylglutamic acid, N10-formylpteroylglutamic acid and citro vorum factor are inhibited non-competitively by a number of 1,2-dihydro-s-triazines when substituted for pteroylglutamic acid in 5. faecalis No. 8043 assay systems, but only by inhibitor:metabolite ratios considerably higher than those required for the inhibition of pteroylglutamic acid in the same microbiological system. Citrovorum factor also is similarly inhibited in L. citrovorum No. 8081 assay systems. The phenyl- and 3′- and 4′- halophenyl-2,2-dimethyl derivatives were the most active compounds of the series and certain correlations can be made between structure and microbiological activity vs these metabolites.

Amino Acid Metabolism in Mammalian Cell Cultures

Abstract: The present article \"is a progress report rather than a review and in large part summarizes studies from a single laboratory\" on the minimal essential medium for cultivation of mammalian cells in either monolayer or suspension. Every cell culture examined, whether human or animal in origin, required at least 13 amino acids for survival and growth. All the cultured human cells examined were found to contain large amounts of glutathione, taurine, glutamine, ammonia, and glutamic acid. [The SCI® indicates that this paper was cited 2,255 times in the period 1961-1975.]

Studies on the mechanism of action of cytoxan. Evidence of activation in vivo and in vitro.

Abstract: Summary The reported inactivity of Cytoxan, when assayed directly in mammalian cell cultures, has been confirmed, even with cell lines derived from experimental tumors which respond in vivo to Cytoxan therapy. However, blood serum or crude liver extracts from rats treated with Cytoxan were inhibitory in vitro for serially propagated mammalian cells derived from normal or neoplastic tissue, as well as for cells in primary culture. Experiments in which Cytoxan was incubated in vitro in homogenates of normal and neoplastic mouse tissue indicate that, of the tissues so examined under the present experimental conditions, only the liver homogenates contained a substance (or substances) inhibitory for mammalian cells in culture. The experimental data presented herein support the hypothesis that Cytoxan is a “transport” structure which becomes biologically active only upon appropriate “activation,” and that “activation” sufficient to interfere with cell growth, as adjudged by the present in vitro methods of assay, is accomplished primarily (but probably not exclusively) by the liver.