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G. H. Sloane Stanley

Bio: G. H. Sloane Stanley is an academic researcher from Harvard University. The author has contributed to research in topics: Simple (abstract algebra) & Triglyceride transport. The author has an hindex of 3, co-authored 3 publications receiving 56897 citations.

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Journal ArticleDOI
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.

59,550 citations

Journal ArticleDOI
TL;DR: ThannHAUSER, FELL~C and SCHMIDT (1955), using THANNHAUSER and BONCODDO'S method of preparation (1953), have shown that the esterification of the sulphuric acid occurs.
Abstract: THE existence of brain lipides which contain sulphur, i.e. sulphatides, has been recognized since the time of THUDICHUM (1901). However, because of the difficulties encountered in their isolation, only one sulphatide has ever been isolated and characterized to any extent, i.e. the potassium salt of cerebron sulphuric acid prepared by BLIX (1933). BLIX found equimolar amounts of cerebronic acid, sphingosine, and galactose in hydrolysates of his compound. Since these were the same constituents as had been found in cerebrosides, he concluded that his compound had the structure of a cerebroside esterified with sulphuric acid (Fig. 1). THANNHAUSER, FELL~C and SCHMIDT (1955), using THANNHAUSER and BONCODDO'S method of preparation (1953), have shown that the esterification of the sulphuric acid occurs

71 citations

01 Jan 1957
TL;DR: A simplified version of the method for the preparation and purification of brain lipides has the advantage of being simpler, of being applicable to any scale desired, of substantially decreasing the losses of lipides incidental to the washing process, and of yielding a washed extract which can be taken to dryness without foaming and without splitting of the proteolipides.

3 citations


Cited by
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Journal ArticleDOI
E. G. Bligh1, W. J. Dyer1
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.
Abstract: Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can...

46,099 citations

Journal ArticleDOI
TL;DR: The procedure developed is simple, rapid, and generally applicable t o lipids, and the results did not affect the validity of the method.

5,749 citations

Journal ArticleDOI
TL;DR: Kinetic analysis indicates that TPA can substitute for diacylglycerol and greatly increases the affinity of the enzyme for Ca2+ as well as for phospholipid, and various phorbol derivatives which have been shown to be active in tumor promotion are also capable of activating this protein kinase in in vitro systems.

4,562 citations

Journal ArticleDOI
TL;DR: The effects of the ionic strength and pH of the hemolyzing solution on the hemoglobin content of human erythrocyte ghosts were studied in phosphate buffers and suggest an electrophysical interaction of hemoglobin with membrane constituents.

4,227 citations

Journal ArticleDOI
07 Apr 2011-Nature
TL;DR: Discovery of a relationship between gut-flora-dependent metabolism of dietary phosphatidylcholine and CVD pathogenesis provides opportunities for the development of new diagnostic tests and therapeutic approaches for atherosclerotic heart disease.
Abstract: Metabolomics studies hold promise for the discovery of pathways linked to disease processes. Cardiovascular disease (CVD) represents the leading cause of death and morbidity worldwide. Here we used a metabolomics approach to generate unbiased small-molecule metabolic profiles in plasma that predict risk for CVD. Three metabolites of the dietary lipid phosphatidylcholine—choline, trimethylamine N-oxide (TMAO) and betaine—were identified and then shown to predict risk for CVD in an independent large clinical cohort. Dietary supplementation of mice with choline, TMAO or betaine promoted upregulation of multiple macrophage scavenger receptors linked to atherosclerosis, and supplementation with choline or TMAO promoted atherosclerosis. Studies using germ-free mice confirmed a critical role for dietary choline and gut flora in TMAO production, augmented macrophage cholesterol accumulation and foam cell formation. Suppression of intestinal microflora in atherosclerosis-prone mice inhibited dietary-choline-enhanced atherosclerosis. Genetic variations controlling expression of flavin monooxygenases, an enzymatic source of TMAO, segregated with atherosclerosis in hyperlipidaemic mice. Discovery of a relationship between gut-flora-dependent metabolism of dietary phosphatidylcholine and CVD pathogenesis provides opportunities for the development of new diagnostic tests and therapeutic approaches for atherosclerotic heart disease.

4,107 citations