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G. L. Miller

Bio: G. L. Miller is an academic researcher. The author has contributed to research in topics: Reducing sugar & 3,5-Dinitrosalicylic acid. The author has an hindex of 1, co-authored 1 publications receiving 23198 citations.

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Book ChapterDOI
C. Stan Tsai1
14 Apr 2006

3,340 citations

Journal ArticleDOI
TL;DR: In this paper, a collaborative investigation of assays for endo-1,4-β-xylanase activity based on production of reducing sugars from polymeric 4-O-methyl glucuronoxylan was conducted.

2,332 citations

Journal ArticleDOI
TL;DR: In this paper, the authors review quantitative cellulase activity assays using soluble and insoluble substrates, and focus on their advantages and limitations, and hypothesize that continuous culture using insoluble cellulosic substrates could be a powerful selection tool for enriching beneficial cellulase mutants from the large library displayed on the cell surface.

1,495 citations

Journal ArticleDOI
TL;DR: The combined results suggested that SNPs may damage the structure of bacterial cell membrane and depress the activity of some membranous enzymes, which cause E. coli bacteria to die eventually.
Abstract: The antibacterial activity and acting mechanism of silver nanoparticles (SNPs) on Escherichia coli ATCC 8739 were investigated in this study by analyzing the growth, permeability, and morphology of the bacterial cells following treatment with SNPs. The experimental results indicated 10 microg/ml SNPs could completely inhibit the growth of 10(7) cfu/ml E. coli cells in liquid Mueller-Hinton medium. Meanwhile, SNPs resulted in the leakage of reducing sugars and proteins and induced the respiratory chain dehydrogenases into inactive state, suggesting that SNPs were able to destroy the permeability of the bacterial membranes. When the cells of E. coli were exposed to 50 microg/ml SNPs, many pits and gaps were observed in bacterial cells by transmission electron microscopy and scanning electron microscopy, and the cell membrane was fragmentary, indicating the bacterial cells were damaged severely. After being exposed to 10 microg/ml SNPs, the membrane vesicles were dissolved and dispersed, and their membrane components became disorganized and scattered from their original ordered and close arrangement based on TEM observation. In conclusion, the combined results suggested that SNPs may damage the structure of bacterial cell membrane and depress the activity of some membranous enzymes, which cause E. coli bacteria to die eventually.

1,326 citations

Journal ArticleDOI
TL;DR: This review focuses on the microbial amylases and their application with a biotechnological perspective and α-Amylase holds the maximum market share of enzyme sales with its major application in the starch industry as well as its well-known usage in bakery.

1,214 citations